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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 619301 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#619301, RRID:AB_2209724
- Product name
- Purified anti-TIF1
- Antibody type
- Monoclonal
- Antigen
- TIF1b
- Description
- TIFβ (transcription intermediary factor 1-beta) is an 88 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits setdbl to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with setdbl and cbx3 proteins. The 20A1 monoclonal antibody has been shown to recognize the human TIF1β protein and has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store at 4° C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-TIF1β antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated
