Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB500-159 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-159, RRID:AB_2209895
- Product name
- Rabbit Polyclonal KAP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The Rad50 hook domain regulates DNA damage signaling and tumorigenesis.
Roset R, Inagaki A, Hohl M, Brenet F, Lafrance-Vanasse J, Lange J, Scandura JM, Tainer JA, Keeney S, Petrini JH
Genes & development 2014 Mar 1;28(5):451-62
Genes & development 2014 Mar 1;28(5):451-62
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KAP1 Antibody [NB500-159] - Detection of Human and Mouse KAP-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody NB500-159 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: KAP1 Antibody [NB500-159] - Whole cell lysate (1 mg for IP;20% of IP loaded) from HeLa cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: KAP1 Antibody [NB500-159] - Detection of human KAP-1 by western blot of immunoprecipitates. Samples Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NB500-159 (lot NB500-159-4) used for IP at 6 ug per reaction. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-159-3) and rabbit anti-KAP-1 antibody NB500-158. For blotting immunoprecipitated KAP-1, NB500-159 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: KAP1 Antibody [NB500-159] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: KAP1 Antibody [NB500-159] - Sample: FFPE section of mouse plasmacytoma Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).