Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB2016-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human S1P1/EDG-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human S1P1/EDG-1 by flow cytometry.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P21453
- Isotype
- IgG
- Antibody clone number
- 218713
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A sphingosine-1-phosphate receptor type 1 agonist, ASP4058, suppresses intracranial aneurysm through promoting endothelial integrity and blocking macrophage transmigration.
Expression of functional sphingosine-1 phosphate receptor-1 is reduced by B cell receptor signaling and increased by inhibition of PI3 kinase δ but not SYK or BTK in chronic lymphocytic leukemia cells.
Sphingosine-1-phosphate (S1P) displays sustained S1P1 receptor agonism and signaling through S1P lyase-dependent receptor recycling.
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
New B-cell CD molecules.
Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
Yamamoto R, Aoki T, Koseki H, Fukuda M, Hirose J, Tsuji K, Takizawa K, Nakamura S, Miyata H, Hamakawa N, Kasuya H, Nozaki K, Hirayama Y, Aramori I, Narumiya S
British journal of pharmacology 2017 Jul;174(13):2085-2101
British journal of pharmacology 2017 Jul;174(13):2085-2101
Expression of functional sphingosine-1 phosphate receptor-1 is reduced by B cell receptor signaling and increased by inhibition of PI3 kinase δ but not SYK or BTK in chronic lymphocytic leukemia cells.
Till KJ, Pettitt AR, Slupsky JR
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 1;194(5):2439-46
Journal of immunology (Baltimore, Md. : 1950) 2015 Mar 1;194(5):2439-46
Sphingosine-1-phosphate (S1P) displays sustained S1P1 receptor agonism and signaling through S1P lyase-dependent receptor recycling.
Gatfield J, Monnier L, Studer R, Bolli MH, Steiner B, Nayler O
Cellular signalling 2014 Jul;26(7):1576-88
Cellular signalling 2014 Jul;26(7):1576-88
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
Cabezón R, Sintes J, Llinàs L, Benitez-Ribas D
Immunology letters 2011 Jan 30;134(2):167-73
Immunology letters 2011 Jan 30;134(2):167-73
New B-cell CD molecules.
Matesanz-Isabel J, Sintes J, Llinàs L, de Salort J, Lázaro A, Engel P
Immunology letters 2011 Jan 30;134(2):104-12
Immunology letters 2011 Jan 30;134(2):104-12
Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noël A, Foidart JM, Munaut C, Péqueux C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Oct;24(10):3782-95
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Oct;24(10):3782-95
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of S1P1/EDG-1 in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 and eGFP by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with either (A) human S1P1/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB2016) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.