Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- MAB7089 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse S1P1/EDG-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse S1P1/EDG-1 peptide in direct ELISAs.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
O08530
- Isotype
- IgG
- Antibody clone number
- 713412
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Macrophages Induce Long-Term Trapping of γδ T Cells with Innate-like Properties within Secondary Lymphoid Organs in the Steady State.
A Coronin 1-Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment.
Lymphatic endothelial S1P promotes mitochondrial function and survival in naive T cells.
CD8αα intraepithelial lymphocytes arise from two main thymic precursors.
Ageing adversely affects the migration and function of marginal zone B cells.
Thymic progenitors of TCRαβ+ CD8αα intestinal intraepithelial lymphocytes require RasGRP1 for development.
IL-17-Producing Vγ4+ γδ T Cells Require Sphingosine 1-Phosphate Receptor 1 for Their Egress from the Lymph Nodes under Homeostatic and Inflammatory Conditions.
Mir-17-92 regulates bone marrow homing of plasma cells and production of immunoglobulin G2c.
Control of lymphocyte egress from lymph nodes through β2-adrenergic receptors.
Moesin controls clathrin-mediated S1PR1 internalization in T cells.
Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress.
The sphingosine 1-phosphate receptor S1P₂ maintains the homeostasis of germinal center B cells and promotes niche confinement.
Audemard-Verger A, Rivière M, Durand A, Peranzoni E, Guichard V, Hamon P, Bonilla N, Guilbert T, Boissonnas A, Auffray C, Eberl G, Lucas B, Martin B
Journal of immunology (Baltimore, Md. : 1950) 2017 Sep 15;199(6):1998-2007
Journal of immunology (Baltimore, Md. : 1950) 2017 Sep 15;199(6):1998-2007
A Coronin 1-Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment.
Lang MJ, Mori M, Ruer-Laventie J, Pieters J
Journal of immunology (Baltimore, Md. : 1950) 2017 Oct 1;199(7):2421-2431
Journal of immunology (Baltimore, Md. : 1950) 2017 Oct 1;199(7):2421-2431
Lymphatic endothelial S1P promotes mitochondrial function and survival in naive T cells.
Mendoza A, Fang V, Chen C, Serasinghe M, Verma A, Muller J, Chaluvadi VS, Dustin ML, Hla T, Elemento O, Chipuk JE, Schwab SR
Nature 2017 Jun 1;546(7656):158-161
Nature 2017 Jun 1;546(7656):158-161
CD8αα intraepithelial lymphocytes arise from two main thymic precursors.
Ruscher R, Kummer RL, Lee YJ, Jameson SC, Hogquist KA
Nature immunology 2017 Jul;18(7):771-779
Nature immunology 2017 Jul;18(7):771-779
Ageing adversely affects the migration and function of marginal zone B cells.
Turner VM, Mabbott NA
Immunology 2017 Jul;151(3):349-362
Immunology 2017 Jul;151(3):349-362
Thymic progenitors of TCRαβ+ CD8αα intestinal intraepithelial lymphocytes require RasGRP1 for development.
Golec DP, Hoeppli RE, Henao Caviedes LM, McCann J, Levings MK, Baldwin TA
The Journal of experimental medicine 2017 Aug 7;214(8):2421-2435
The Journal of experimental medicine 2017 Aug 7;214(8):2421-2435
IL-17-Producing Vγ4+ γδ T Cells Require Sphingosine 1-Phosphate Receptor 1 for Their Egress from the Lymph Nodes under Homeostatic and Inflammatory Conditions.
Maeda Y, Seki N, Kataoka H, Takemoto K, Utsumi H, Fukunari A, Sugahara K, Chiba K
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1408-16
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1408-16
Mir-17-92 regulates bone marrow homing of plasma cells and production of immunoglobulin G2c.
Xu S, Ou X, Huo J, Lim K, Huang Y, Chee S, Lam KP
Nature communications 2015 Apr 17;6:6764
Nature communications 2015 Apr 17;6:6764
Control of lymphocyte egress from lymph nodes through β2-adrenergic receptors.
Nakai A, Hayano Y, Furuta F, Noda M, Suzuki K
The Journal of experimental medicine 2014 Dec 15;211(13):2583-98
The Journal of experimental medicine 2014 Dec 15;211(13):2583-98
Moesin controls clathrin-mediated S1PR1 internalization in T cells.
Nomachi A, Yoshinaga M, Liu J, Kanchanawong P, Tohyama K, Thumkeo D, Watanabe T, Narumiya S, Hirata T
PloS one 2013;8(12):e82590
PloS one 2013;8(12):e82590
Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress.
Arnon TI, Horton RM, Grigorova IL, Cyster JG
Nature 2013 Jan 31;493(7434):684-8
Nature 2013 Jan 31;493(7434):684-8
The sphingosine 1-phosphate receptor S1P₂ maintains the homeostasis of germinal center B cells and promotes niche confinement.
Green JA, Suzuki K, Cho B, Willison LD, Palmer D, Allen CD, Schmidt TH, Xu Y, Proia RL, Coughlin SR, Cyster JG
Nature immunology 2011 Jun 5;12(7):672-80
Nature immunology 2011 Jun 5;12(7):672-80
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- S1P1/EDG-1 in Mouse Heart. S1P1/EDG-1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of S1P1/EDG-1 in HEK293 Human Cell Line Transfected With S1P1/EDG-1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of S1P1/EDG-1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.