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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00001901-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001901-M03, RRID:AB_1111802
- Product name
- EDG1 monoclonal antibody (M03), clone 1F11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant EDG1.
- Antigen sequence
MGPTSVPLVKAHRSSVSDYVNYDIIVRHYNYTGKL
NISADKENSIKL- Isotype
- IgG
- Antibody clone number
- 1F11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of EDG1 expression in transfected 293T cell line by EDG1 monoclonal antibody (M03), clone 1F11.Lane 1: EDG1 transfected lysate(42.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged EDG1 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
