Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 50-3639-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD363 (S1PR1) Monoclonal Antibody (SW4GYPP), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The SW4GYPP monoclonal antibody recognizes human CD363, also known as sphingosine-1-phosphate receptor 1 (S1PR1, S1P1), or endothelial differentiation G-protein coupled receptor 1 (EDG1). CD363 is a part of a family of five G-protein-coupled receptors that bind sphingosine-1-phosphate (S1P), a lysophospholipid mediator. It has been shown to be primarily expressed by lymphocytes but is also seen on endothelial cells, B cells, NK cells, monocytes/macrophages, neural progenitors, neurons, astrocytes, and oligodendrocytes. CD363 surface expression is dependent on the extracellular concentration of S1P and its signaling is important for lymphocyte egress from secondary lymphoid tissues and the thymus. CD363 plays a key role in many cellular processes like angiogenesis, vascular maturation, endothelial barrier function and trafficking of immune cells. Therapeutic CD363 modulators have been successful in the treatment of relapsing-remitting multiple sclerosis through sequestration of immune cells in secondary lymphoid organs and, therefore, away from sites of inflammation. Applications Reported: This SW4GYPP antibody has been reported for use in flow cytometric analysis. Applications Tested: This SW4GYPP antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SW4GYPP
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Heterozygous premature termination in zinc-finger domain of Krüppel-like factor 2 gene associates with dysregulated immunity.
Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa.
Detection of HIV-1-specific gastrointestinal tissue resident CD8(+) T-cells in chronic infection.
Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming.
Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis.
Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.
"Inside-out" signaling of sphingosine-1-phosphate: therapeutic targets.
Migration of CD4 T cells and dendritic cells toward sphingosine 1-phosphate (S1P) is mediated by different receptor subtypes: S1P regulates the functions of murine mature dendritic cells via S1P receptor type 3.
The immunosuppressant FTY720 down-regulates sphingosine 1-phosphate G-protein-coupled receptors.
Pernaa N, Keskitalo S, Chowdhury I, Nissinen A, Glumoff V, Keski-Filppula R, Junttila J, Eklund KK, Santaniemi W, Siitonen S, Seppänen MR, Vähäsalo P, Varjosalo M, Åström P, Hautala T
Frontiers in immunology 2022;13:819929
Frontiers in immunology 2022;13:819929
Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa.
Cantero-Pérez J, Grau-Expósito J, Serra-Peinado C, Rosero DA, Luque-Ballesteros L, Astorga-Gamaza A, Castellví J, Sanhueza T, Tapia G, Lloveras B, Fernández MA, Prado JG, Solé-Sedeno JM, Tarrats A, Lecumberri C, Mañalich-Barrachina L, Centeno-Mediavilla C, Falcó V, Buzon MJ, Genescà M
Nature communications 2019 Oct 18;10(1):4739
Nature communications 2019 Oct 18;10(1):4739
Detection of HIV-1-specific gastrointestinal tissue resident CD8(+) T-cells in chronic infection.
Kiniry BE, Li S, Ganesh A, Hunt PW, Somsouk M, Skinner PJ, Deeks SG, Shacklett BL
Mucosal immunology 2018 May;11(3):909-920
Mucosal immunology 2018 May;11(3):909-920
Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming.
Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, Tregnago C, Corrado M, Pigazzi M, Barnaba V, Tripodo C, Matarese G, Piconese S, Campello S
Cell reports 2018 Dec 11;25(11):3059-3073.e10
Cell reports 2018 Dec 11;25(11):3059-3073.e10
Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis.
Ickrath P, Kleinsasser N, Ding X, Ginzkey C, Beyersdorf N, Hagen R, Kerkau T, Hackenberg S
International journal of molecular medicine 2018 Aug;42(2):1116-1124
International journal of molecular medicine 2018 Aug;42(2):1116-1124
Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.
Cuff AO, Robertson FP, Stegmann KA, Pallett LJ, Maini MK, Davidson BR, Male V
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4283-4291
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4283-4291
"Inside-out" signaling of sphingosine-1-phosphate: therapeutic targets.
Takabe K, Paugh SW, Milstien S, Spiegel S
Pharmacological reviews 2008 Jun;60(2):181-95
Pharmacological reviews 2008 Jun;60(2):181-95
Migration of CD4 T cells and dendritic cells toward sphingosine 1-phosphate (S1P) is mediated by different receptor subtypes: S1P regulates the functions of murine mature dendritic cells via S1P receptor type 3.
Maeda Y, Matsuyuki H, Shimano K, Kataoka H, Sugahara K, Chiba K
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 15;178(6):3437-46
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 15;178(6):3437-46
The immunosuppressant FTY720 down-regulates sphingosine 1-phosphate G-protein-coupled receptors.
Gräler MH, Goetzl EJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2004 Mar;18(3):551-3
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2004 Mar;18(3):551-3
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0047-42) and Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or Anti-Human CD363 (S1PR1) eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.