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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-519 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-519, RRID:AB_632073
- Product name
- Anti-NRAS
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Rapid and selective detection of fatty acylated proteins using omega-alkynyl-fatty acids and click chemistry.
Analysis of k-ras nuclear expression in fibroblasts and mesangial cells.
Concordant regulation of translation and mRNA abundance for hundreds of targets of a human microRNA.
Transcriptional networks of knockout cell lines identify functional specificities of H-Ras and N-Ras: significant involvement of N-Ras in biotic and defense responses
Targeted genomic disruption of H-ras and N-ras, individually or in combination, reveals the dispensability of both loci for mouse growth and development.
Yap MC, Kostiuk MA, Martin DD, Perinpanayagam MA, Hak PG, Siddam A, Majjigapu JR, Rajaiah G, Keller BO, Prescher JA, Wu P, Bertozzi CR, Falck JR, Berthiaume LG
Journal of lipid research 2010 Jun;51(6):1566-80
Journal of lipid research 2010 Jun;51(6):1566-80
Analysis of k-ras nuclear expression in fibroblasts and mesangial cells.
Fuentes-Calvo I, Blázquez-Medela AM, Santos E, López-Novoa JM, Martínez-Salgado C
PloS one 2010 Jan 14;5(1):e8703
PloS one 2010 Jan 14;5(1):e8703
Concordant regulation of translation and mRNA abundance for hundreds of targets of a human microRNA.
Hendrickson DG, Hogan DJ, McCullough HL, Myers JW, Herschlag D, Ferrell JE, Brown PO
PLoS biology 2009 Nov;7(11):e1000238
PLoS biology 2009 Nov;7(11):e1000238
Transcriptional networks of knockout cell lines identify functional specificities of H-Ras and N-Ras: significant involvement of N-Ras in biotic and defense responses
E Castellano, J De Las Rivas, C Guerrero, E Santos
Oncogene 2006 Aug;26(6):917-933
Oncogene 2006 Aug;26(6):917-933
Targeted genomic disruption of H-ras and N-ras, individually or in combination, reveals the dispensability of both loci for mouse growth and development.
Esteban LM, Vicario-Abejón C, Fernández-Salguero P, Fernández-Medarde A, Swaminathan N, Yienger K, Lopez E, Malumbres M, McKay R, Ward JM, Pellicer A, Santos E
Molecular and cellular biology 2001 Mar;21(5):1444-52
Molecular and cellular biology 2001 Mar;21(5):1444-52
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 213, 118, 83, 50, 33, 26, 18
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 21
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
