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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA505835 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA505835, RRID:AB_2623403
- Product name
- NRAS mouse monoclonal antibody, clone OTI5G7 (formerly 5G7)
- Antibody type
- Monoclonal
- Description
- NRAS mouse monoclonal antibody, clone OTI5G7 (formerly 5G7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NRAS
- Isotype
- IgG
- Antibody clone number
- OTI5G7
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Usp9x regulates Ets-1 ubiquitination and stability to control NRAS expression and tumorigenicity in melanoma.
Potu H, Peterson LF, Kandarpa M, Pal A, Sun H, Durham A, Harms PW, Hollenhorst PC, Eskiocak U, Talpaz M, Donato NJ
Nature communications 2017 Feb 15;8:14449
Nature communications 2017 Feb 15;8:14449
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRAS (RC202681, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRAS.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505835)
- Validation comment
- IHC
