Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- GTX132403 - Provider product page
- Provider
- GeneTex
- Product name
- Ras (A59T Mutant Specific) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Ras (A59T Mutant specific) antibody detects Ras (A59T Mutant specific) protein by western blot analysis. Non-transfected (-) and Ras (A59T Mutant specific)-transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (A59T Mutant specific) antibody (GTX132403) diluted at 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (A59T Mutant Specific) antibody (GTX132403) and HA tag antibody [GT423] (GTX628489) diluted at 1:5000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (A59T Mutant specific) antibody (GTX132403) diluted at 1:1000.