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- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Flow cytometry [2]
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- Product number
- F50733 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NRAS Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific NRAS antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence/ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the NRAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of NRAS antibody and CEM lysate. Predicted molecular weight ~21 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of NRAS antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the NRAS gene (2).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of NRAS antibody and CEM lysate. Predicted molecular weight ~21 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of 1) human HepG2, 2) mouse testis and 3) rat lung tissue lysate with NRAS antibody. Predicted molecular weight ~21 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of human 1) A431 and 2) HepG2 cell lysate with NRAS antibody. Predicted molecular weight ~21 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of NRAS antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of NRAS antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- NRAS antibody flow cytometric analysis of NCI-H460 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- NRAS antibody flow cytometric analysis of NCI-H460 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
