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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- AM09391PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM09391PU-N, RRID:AB_10978549
- Product name
- anti RAS
- Antibody type
- Monoclonal
- Antigen
- Recombinant human NRAS (1-186aa) purified from E. coli
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AT2G9
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- The extracts of 293T(35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cross-reacting of anti-human RAS(AT2G9) was analyzed by western blot with recombinant protein (200ng) of NRAS, KRAS and HRAS.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: The extracts of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The cross-reacting of anti-human RAS (AT2G9) was analyzed by Western blot with recombinant protein (200ng) of NRAS, KRAS and HRAS.
