Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [2]
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Validation data
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- Product number
- NB100-463 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-463, RRID:AB_10002437
- Product name
- Rabbit Polyclonal TTK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Phase I clinical study of multiple epitope peptide vaccine combined with chemoradiation therapy in esophageal cancer patients.
Iinuma H, Fukushima R, Inaba T, Tamura J, Inoue T, Ogawa E, Horikawa M, Ikeda Y, Matsutani N, Takeda K, Yoshida K, Tsunoda T, Ikeda T, Nakamura Y, Okinaga K
Journal of translational medicine 2014 Apr 3;12:84
Journal of translational medicine 2014 Apr 3;12:84
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TTK Antibody [NB100-463] - TTK protein kinase Antibody [NB100-463] - Detection of human TTK in 15 or 50 ug of HeLa whole cell lysate and in HeLa cells treated with an siRNA for vimentin (control) or for TTK (siTTK), using NB 100-463.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TTK Antibody [NB100-463] - Detection of human TTK by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-TTK antibody NB100-463 used for IP at 6 ug per reaction. TTK was also immunoprecipitated by another rabbit anti-TTK antibody. Detection: Chemiluminescence with an exposure time of 3 minutes.