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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- RQ4517 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- XPO1 Antibody / Exportin 1 / CRM1
- Antibody type
- Monoclonal
- Description
- This highly specific XPO1 antibody is suitable for use in Immunohistochemistry/Western blot/Flow cytometry/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 5G3
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the XPO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) rat lung, 3) mouse liver, 4) mouse lung, 5) rabbit IgG, 6) molecular weight marker, 7) human HepG2, 8) human SMMC-7721, 9) human HeLa and 10) human Jurkat lysate with XPO1 antibody at 0.5ug/ml. Expected molecular weight ~123 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lung cancer with XPO1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human intestinal cancer with XPO1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
