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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- 600-401-A49 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-A49, RRID:AB_2126104
- Product name
- Anti-IL-7 Receptor alpha chain pY449 (RABBIT) Antibody - 600-401-A49
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Salmonella Activation of STAT3 Signaling by SarA Effector Promotes Intracellular Replication and Production of IL-10.
HIV-1 tat protein recruits CIS to the cytoplasmic tail of CD127 to induce receptor ubiquitination and proteasomal degradation.
Jaslow SL, Gibbs KD, Fricke WF, Wang L, Pittman KJ, Mammel MK, Thaden JT, Fowler VG Jr, Hammer GE, Elfenbein JR, Ko DC
Cell reports 2018 Jun 19;23(12):3525-3536
Cell reports 2018 Jun 19;23(12):3525-3536
HIV-1 tat protein recruits CIS to the cytoplasmic tail of CD127 to induce receptor ubiquitination and proteasomal degradation.
Sugden S, Ghazawi F, MacPherson P
Virology 2016 Nov;498:192-200
Virology 2016 Nov;498:192-200
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot and immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4°C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.
- Validation comment
- Western Blot
