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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Blocking/Neutralizing [1]
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- Product number
- AF-249-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human LIFR alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LIF R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 7% cross-reactivity with recombinant mouse LIF R alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P42702- Isotype
- IgG
- Vial size
- 250 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The Phosphoinositide 3-Kinase p110α Isoform Regulates Leukemia Inhibitory Factor Receptor Expression via c-Myc and miR-125b to Promote Cell Proliferation in Medulloblastoma.
A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor.
Disturbances in the LIF pathway in the endometrium among women with unexplained infertility.
Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells.
Receptor-mediated transport of LIF across blood-spinal cord barrier is upregulated after spinal cord injury.
Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression.
The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries.
Leukocytes, cytokines, growth factors and hormones in human skeletal muscle and blood after uphill or downhill running.
Leukemia inhibitory factor regulates prolactin secretion in prolactinoma and lactotroph cells.
Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
Salm F, Dimitrova V, von Bueren AO, Ćwiek P, Rehrauer H, Djonov V, Anderle P, Arcaro A
PloS one 2015;10(4):e0123958
PloS one 2015;10(4):e0123958
A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor.
Chollangi S, Mather T, Rodgers KK, Ash JD
The Journal of biological chemistry 2012 Sep 21;287(39):32848-59
The Journal of biological chemistry 2012 Sep 21;287(39):32848-59
Disturbances in the LIF pathway in the endometrium among women with unexplained infertility.
Aghajanova L, Altmäe S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A
Fertility and sterility 2009 Jun;91(6):2602-10
Fertility and sterility 2009 Jun;91(6):2602-10
Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells.
Aghajanova L, Skottman H, Strömberg AM, Inzunza J, Lahesmaa R, Hovatta O
Fertility and sterility 2006 Oct;86(4 Suppl):1193-209
Fertility and sterility 2006 Oct;86(4 Suppl):1193-209
Receptor-mediated transport of LIF across blood-spinal cord barrier is upregulated after spinal cord injury.
Pan W, Cain C, Yu Y, Kastin AJ
Journal of neuroimmunology 2006 May;174(1-2):119-25
Journal of neuroimmunology 2006 May;174(1-2):119-25
Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression.
Queen MM, Ryan RE, Holzer RG, Keller-Peck CR, Jorcyk CL
Cancer research 2005 Oct 1;65(19):8896-904
Cancer research 2005 Oct 1;65(19):8896-904
The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
He W, Gong K, Smith DK, Ip NY
FEBS letters 2005 Aug 15;579(20):4317-23
FEBS letters 2005 Aug 15;579(20):4317-23
Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries.
Abir R, Fisch B, Jin S, Barnnet M, Freimann S, Van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A
Molecular human reproduction 2004 May;10(5):313-9
Molecular human reproduction 2004 May;10(5):313-9
Leukocytes, cytokines, growth factors and hormones in human skeletal muscle and blood after uphill or downhill running.
Malm C, Sjödin TL, Sjöberg B, Lenkei R, Renström P, Lundberg IE, Ekblom B
The Journal of physiology 2004 May 1;556(Pt 3):983-1000
The Journal of physiology 2004 May 1;556(Pt 3):983-1000
Leukemia inhibitory factor regulates prolactin secretion in prolactinoma and lactotroph cells.
Ben-Shlomo A, Miklovsky I, Ren SG, Yong WH, Heaney AP, Culler MD, Melmed S
The Journal of clinical endocrinology and metabolism 2003 Feb;88(2):858-63
The Journal of clinical endocrinology and metabolism 2003 Feb;88(2):858-63
Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
He W, Gong K, Zhu G, Smith DK, Ip NY
FEBS letters 2002 Mar 13;514(2-3):214-8
FEBS letters 2002 Mar 13;514(2-3):214-8
Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
He W, Gong K, Zhu G, Smith DK, Ip NY
FEBS letters 2002 Mar 13;514(2-3):214-8
FEBS letters 2002 Mar 13;514(2-3):214-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by LIF and Neutralization by Human LIF R alpha Antibody. Recombinant Human LIF stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by 0.3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-249-NA). The ND50 is typically 6-36 µg/mL.
