MA5-13799

antibody from Invitrogen Antibodies

Targeting: IGF1R CD221, IGFIR, IGFR, JTK13, MGC18216

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-13799

Provider

Invitrogen Antibodies

Product name

IGF1R alpha Monoclonal Antibody (24-31), Biotin

Antibody type

Monoclonal

Antigen

Other

Description

MA5-13799 targets IGF-1 Receptor alpha in ELISA, ICC/IF, IHC (P) and immunoprecipitation applications and shows reactivity with Human samples.

Conjugate

Biotin

Antibody clone number

24-31

Concentration

0.2 mg/mL

References

Submitted references

Multiplexed Exchange-PAINT imaging reveals ligand-dependent EGFR and Met interactions in the plasma membrane.

Werbin JL, Avendaño MS, Becker V, Jungmann R, Yin P, Danuser G, Sorger PK
Scientific reports 2017 Sep 22;7(1):12150

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Insulin-like growth factor 1 receptor (IGF1R) expression and survival in operable squamous-cell laryngeal cancer.

Mountzios G, Kostopoulos I, Kotoula V, Sfakianaki I, Fountzilas E, Markou K, Karasmanis I, Leva S, Angouridakis N, Vlachtsis K, Nikolaou A, Konstantinidis I, Fountzilas G
PloS one 2013;8(1):e54048

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Does insulin-like growth factor 1 receptor (IGF-1R) targeting provide new treatment options for chordomas? A retrospective clinical and immunohistochemical study.

Scheipl S, Froehlich EV, Leithner A, Beham A, Quehenberger F, Mokry M, Stammberger H, Varga PP, Lazáry A, Windhager R, Gattenloehner S, Liegl B
Histopathology 2012 May;60(6):999-1003

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Utility of insulin-like growth factor receptor-1 expression in gefitinib-treated patients with non-small cell lung cancer.

Fidler MJ, Basu S, Buckingham L, Walters K, McCormack S, Batus M, Coon J 4th, Bonomi P
Anticancer research 2012 May;32(5):1705-10

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Phase II study of preoperative systemic treatment with the combination of docetaxel and trastuzumab in patients with locally advanced HER-2-overexpressing breast cancer.

Sawaki M, Iwata H, Sato Y, Wada M, Toyama T, Sasaki E, Yatabe Y, Imai T, Ohashi Y
Breast (Edinburgh, Scotland) 2010 Oct;19(5):370-6

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Relationships of insulin-like growth factor-1 receptor and epidermal growth factor receptor expression to clinical outcomes in patients with colorectal cancer.

Takahari D, Yamada Y, Okita NT, Honda T, Hirashima Y, Matsubara J, Takashima A, Kato K, Hamaguchi T, Shirao K, Shimada Y, Shimoda T
Oncology 2009;76(1):42-8

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High coexpression of both insulin-like growth factor receptor-1 (IGFR-1) and epidermal growth factor receptor (EGFR) is associated with shorter disease-free survival in resected non-small-cell lung cancer patients.

Ludovini V, Bellezza G, Pistola L, Bianconi F, Di Carlo L, Sidoni A, Semeraro A, Del Sordo R, Tofanetti FR, Mameli MG, Daddi G, Cavaliere A, Tonato M, Crinò L
Annals of oncology : official journal of the European Society for Medical Oncology 2009 May;20(5):842-9

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Impact of insulin-like growth factor type 1 receptor, epidermal growth factor receptor, and HER2 expressions on outcomes of patients with gastric cancer.

Matsubara J, Yamada Y, Hirashima Y, Takahari D, Okita NT, Kato K, Hamaguchi T, Shirao K, Shimada Y, Shimoda T
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 May 15;14(10):3022-9

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HoxA9 induces insulin-like growth factor-1 receptor expression in B-lineage acute lymphoblastic leukemia.

Whelan JT, Ludwig DL, Bertrand FE
Leukemia 2008 Jun;22(6):1161-9

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Predictors of resistance to preoperative trastuzumab and vinorelbine for HER2-positive early breast cancer.

Harris LN, You F, Schnitt SJ, Witkiewicz A, Lu X, Sgroi D, Ryan PD, Come SE, Burstein HJ, Lesnikoski BA, Kamma M, Friedman PN, Gelman R, Iglehart JD, Winer EP
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Feb 15;13(4):1198-207

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Intratumoral IGF-I protein expression is selectively upregulated in breast cancer patients with BRCA1/2 mutations.

Hudelist G, Wagner T, Rosner M, Fink-Retter A, Gschwantler-Kaulich D, Czerwenka K, Kroiss R, Tea M, Pischinger K, Köstler WJ, Attems J, Mueller R, Blaukopf C, Kubista E, Hengstschläger M, Singer CF
Endocrine-related cancer 2007 Dec;14(4):1053-62

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Semi-quantitative scoring of potentially predictive markers for endocrine treatment of breast cancer: a comparison between whole sections and tissue microarrays.

Henriksen KL, Rasmussen BB, Lykkesfeldt AE, Møller S, Ejlertsen B, Mouridsen HT
Journal of clinical pathology 2007 Apr;60(4):397-404

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The p53 pathway and outcome among patients with T1G3 bladder tumors.

López-Knowles E, Hernández S, Kogevinas M, Lloreta J, Amorós A, Tardón A, Carrato A, Kishore S, Serra C, Malats N, Real FX, EPICURO Study Investigators
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Oct 15;12(20 Pt 1):6029-36

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Multiple bronchioloalveolar carcinomas in acromegaly: a potential role of insulin-like growth factor I in carcinogenesis.

Motoi N, Kishi K, Fujii T, Tsuboi E, Ohashi K, Yoshimura K
Lung cancer (Amsterdam, Netherlands) 2006 Nov;54(2):247-53

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Increased hepatic expression of insulin-like growth factor-I receptor in chronic hepatitis C.

Stefano JT, Correa-Giannella ML, Ribeiro CM, Alves VA, Massarollo PC, Machado MC, Giannella-Neto D
World journal of gastroenterology 2006 Jun 28;12(24):3821-8

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Increased hepatic expression of insulin-like growth factor-I receptor in chronic hepatitis C.

Stefano JT, Correa-Giannella ML, Ribeiro CM, Alves VA, Massarollo PC, Machado MC, Giannella-Neto D
World journal of gastroenterology 2006 Jun 28;12(24):3821-8

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Insulin-like growth factor-1 receptor (IGF-1R) expression does not predict for resistance to trastuzumab-based treatment in patients with Her-2/neu overexpressing metastatic breast cancer.

Köstler WJ, Hudelist G, Rabitsch W, Czerwenka K, Müller R, Singer CF, Zielinski CC
Journal of cancer research and clinical oncology 2006 Jan;132(1):9-18

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Retinoblastoma susceptibility gene product pRB activates hypoxia-inducible factor-1 (HIF-1).

Budde A, Schneiderhan-Marra N, Petersen G, Brüne B
Oncogene 2005 Mar 3;24(10):1802-8

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The pT1a and pT1b category subdivision in renal cell carcinoma: is it reflected by differences in tumour biology?

Langner C, Ratschek M, Rehak P, Tsybrovskyy O, Zigeuner R
BJU international 2005 Feb;95(3):310-4

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The role of beta-catenin, TGF beta 3, NGF2, FGF2, IGFR2, and BMP4 in the pathogenesis of mesenteric sclerosis and angiopathy in midgut carcinoids.

Zhang PJ, Furth EE, Cai X, Goldblum JR, Pasha TL, Min KW
Human pathology 2004 Jun;35(6):670-4

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The role of beta-catenin, TGF beta 3, NGF2, FGF2, IGFR2, and BMP4 in the pathogenesis of mesenteric sclerosis and angiopathy in midgut carcinoids.

Zhang PJ, Furth EE, Cai X, Goldblum JR, Pasha TL, Min KW
Human pathology 2004 Jun;35(6):670-4

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Analysis of insulin-like growth factors and insulin-like growth factor I receptor expression in renal cell carcinoma.

Schips L, Zigeuner R, Ratschek M, Rehak P, Rüschoff J, Langner C
American journal of clinical pathology 2004 Dec;122(6):931-7

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Expression of tyrosine kinases in human malignancies as potential targets for kinase-specific inhibitors.

Singer CF, Hudelist G, Lamm W, Mueller R, Czerwenka K, Kubista E
Endocrine-related cancer 2004 Dec;11(4):861-9

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of IGF-1 Receptor alpha (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an IGF-1 Receptor alpha monoclonal antibody (Product # MA5-13802) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Conjugate

Biotin

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Biotin