Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AHR0321 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGF1R alpha Monoclonal Antibody (24-31)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with an epitope at aa 283-440 (exon 4-6).
- Antibody clone number
- 24-31
- Concentration
- 0.2 mg/mL
Submitted references CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities.
Fluorescent-Antibody Targeting of Insulin-Like Growth Factor-1 Receptor Visualizes Metastatic Human Colon Cancer in Orthotopic Mouse Models.
Targeting the insulin growth factor-1 receptor with fluorescent antibodies enables high resolution imaging of human pancreatic cancer in orthotopic mouse models.
Differential expression of the insulin-like growth factor receptor among early breast cancer subtypes.
Metabolic syndrome and colorectal cancer: is hyperinsulinemia/insulin receptor-mediated angiogenesis a critical process?
Increased signalling of EGFR and IGF1R, and deregulation of PTEN/PI3K/Akt pathway are related with trastuzumab resistance in HER2 breast carcinomas.
An ER activity profile including ER, PR, Bcl-2 and IGF-IR may have potential as selection criterion for letrozole or tamoxifen treatment of patients with advanced breast cancer.
The pT1a and pT1b category subdivision in renal cell carcinoma: is it reflected by differences in tumour biology?
Han K, Pierce SE, Li A, Spees K, Anderson GR, Seoane JA, Lo YH, Dubreuil M, Olivas M, Kamber RA, Wainberg M, Kostyrko K, Kelly MR, Yousefi M, Simpkins SW, Yao D, Lee K, Kuo CJ, Jackson PK, Sweet-Cordero A, Kundaje A, Gentles AJ, Curtis C, Winslow MM, Bassik MC
Nature 2020 Apr;580(7801):136-141
Nature 2020 Apr;580(7801):136-141
Fluorescent-Antibody Targeting of Insulin-Like Growth Factor-1 Receptor Visualizes Metastatic Human Colon Cancer in Orthotopic Mouse Models.
Park JY, Murakami T, Lee JY, Zhang Y, Hoffman RM, Bouvet M
PloS one 2016;11(1):e0146504
PloS one 2016;11(1):e0146504
Targeting the insulin growth factor-1 receptor with fluorescent antibodies enables high resolution imaging of human pancreatic cancer in orthotopic mouse models.
Park JY, Lee JY, Zhang Y, Hoffman RM, Bouvet M
Oncotarget 2016 Apr 5;7(14):18262-8
Oncotarget 2016 Apr 5;7(14):18262-8
Differential expression of the insulin-like growth factor receptor among early breast cancer subtypes.
Mountzios G, Aivazi D, Kostopoulos I, Kourea HP, Kouvatseas G, Timotheadou E, Zebekakis P, Efstratiou I, Gogas H, Vamvouka C, Chrisafi S, Stofas A, Pentheroudakis G, Koutras A, Galani E, Bafaloukos D, Fountzilas G
PloS one 2014;9(3):e91407
PloS one 2014;9(3):e91407
Metabolic syndrome and colorectal cancer: is hyperinsulinemia/insulin receptor-mediated angiogenesis a critical process?
Liu JJ, Druta M, Shibata D, Coppola D, Boler I, Elahi A, Reich RR, Siegel E, Extermann M
Journal of geriatric oncology 2014 Jan;5(1):40-8
Journal of geriatric oncology 2014 Jan;5(1):40-8
Increased signalling of EGFR and IGF1R, and deregulation of PTEN/PI3K/Akt pathway are related with trastuzumab resistance in HER2 breast carcinomas.
Gallardo A, Lerma E, Escuin D, Tibau A, Muñoz J, Ojeda B, Barnadas A, Adrover E, Sánchez-Tejada L, Giner D, Ortiz-Martínez F, Peiró G
British journal of cancer 2012 Apr 10;106(8):1367-73
British journal of cancer 2012 Apr 10;106(8):1367-73
An ER activity profile including ER, PR, Bcl-2 and IGF-IR may have potential as selection criterion for letrozole or tamoxifen treatment of patients with advanced breast cancer.
Henriksen KL, Rasmussen BB, Lykkesfeldt AE, Møller S, Ejlertsen B, Mouridsen HT
Acta oncologica (Stockholm, Sweden) 2009;48(4):522-31
Acta oncologica (Stockholm, Sweden) 2009;48(4):522-31
The pT1a and pT1b category subdivision in renal cell carcinoma: is it reflected by differences in tumour biology?
Langner C, Ratschek M, Rehak P, Tsybrovskyy O, Zigeuner R
BJU international 2005 Feb;95(3):310-4
BJU international 2005 Feb;95(3):310-4
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IGF-1 Receptor alpha (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an IGF-1 Receptor alpha monoclonal antibody (Product # AHR0321) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
