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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB391-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse IGF-I R/IGF1R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IGF-I R/IGF1R in sandwich ELISAs and Western blots. Detects mouse IGF-I R/IGF1R in Immunohistochemistry. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha , rhIL-9 R, and rhTGF-beta RII.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P08069- Isotype
- IgG
- Antibody clone number
- 33255
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs.
IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer.
Impact of the putative cancer stem cell markers and growth factor receptor expression on the sensitivity of ovarian cancer cells to treatment with various forms of small molecule tyrosine kinase inhibitors and cytotoxic drugs.
A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes.
Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.
Insulin-like growth factor 1 and 2 (IGF1, IGF2) expression in human microglia: differential regulation by inflammatory mediators.
Targeting both IGF-1R and mTOR synergistically inhibits growth of renal cell carcinoma in vitro.
Proinflammatory actions of visfatin/nicotinamide phosphoribosyltransferase (Nampt) involve regulation of insulin signaling pathway and Nampt enzymatic activity.
Expression of the IGF axis is decreased in local prostate cancer but enhanced after benign prostate epithelial differentiation and TGF-β treatment.
Autologous bone marrow stromal cells genetically engineered to secrete an igf-I receptor decoy prevent the growth of liver metastases.
Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes.
Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
Insulin-like growth factor (IGF) 2 stimulates steroidogenesis and mitosis of bovine granulosa cells through the IGF1 receptor: role of follicle-stimulating hormone and IGF2 receptor.
Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
The susceptibility of granulosa cells to apoptosis is influenced by oestradiol and the cell cycle.
Li J, Weber E, Guth-Gundel S, Schuleit M, Kuttler A, Halleux C, Accart N, Doelemeyer A, Basler A, Tigani B, Wuersch K, Fornaro M, Kneissel M, Stafford A, Freedman BR, Mooney DJ
Advanced healthcare materials 2018 May;7(9):e1701393
Advanced healthcare materials 2018 May;7(9):e1701393
IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer.
Neuzillet Y, Chapeaublanc E, Krucker C, De Koning L, Lebret T, Radvanyi F, Bernard-Pierrot I
BMC cancer 2017 Sep 7;17(1):636
BMC cancer 2017 Sep 7;17(1):636
Impact of the putative cancer stem cell markers and growth factor receptor expression on the sensitivity of ovarian cancer cells to treatment with various forms of small molecule tyrosine kinase inhibitors and cytotoxic drugs.
Puvanenthiran S, Essapen S, Seddon AM, Modjtahedi H
International journal of oncology 2016 Nov;49(5):1825-1838
International journal of oncology 2016 Nov;49(5):1825-1838
A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes.
Gschweitl M, Ulbricht A, Barnes CA, Enchev RI, Stoffel-Studer I, Meyer-Schaller N, Huotari J, Yamauchi Y, Greber UF, Helenius A, Peter M
eLife 2016 Mar 23;5:e13841
eLife 2016 Mar 23;5:e13841
Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.
Garwood CJ, Ratcliffe LE, Morgan SV, Simpson JE, Owens H, Vazquez-Villaseñor I, Heath PR, Romero IA, Ince PG, Wharton SB
Molecular brain 2015 Aug 22;8:51
Molecular brain 2015 Aug 22;8:51
Insulin-like growth factor 1 and 2 (IGF1, IGF2) expression in human microglia: differential regulation by inflammatory mediators.
Suh HS, Zhao ML, Derico L, Choi N, Lee SC
Journal of neuroinflammation 2013 Mar 12;10:37
Journal of neuroinflammation 2013 Mar 12;10:37
Targeting both IGF-1R and mTOR synergistically inhibits growth of renal cell carcinoma in vitro.
Cardillo TM, Trisal P, Arrojo R, Goldenberg DM, Chang CH
BMC cancer 2013 Apr 1;13:170
BMC cancer 2013 Apr 1;13:170
Proinflammatory actions of visfatin/nicotinamide phosphoribosyltransferase (Nampt) involve regulation of insulin signaling pathway and Nampt enzymatic activity.
Jacques C, Holzenberger M, Mladenovic Z, Salvat C, Pecchi E, Berenbaum F, Gosset M
The Journal of biological chemistry 2012 Apr 27;287(18):15100-8
The Journal of biological chemistry 2012 Apr 27;287(18):15100-8
Expression of the IGF axis is decreased in local prostate cancer but enhanced after benign prostate epithelial differentiation and TGF-β treatment.
Massoner P, Ladurner Rennau M, Heidegger I, Kloss-Brandstätter A, Summerer M, Reichhart E, Schäfer G, Klocker H
The American journal of pathology 2011 Dec;179(6):2905-19
The American journal of pathology 2011 Dec;179(6):2905-19
Autologous bone marrow stromal cells genetically engineered to secrete an igf-I receptor decoy prevent the growth of liver metastases.
Wang N, Fallavollita L, Nguyen L, Burnier J, Rafei M, Galipeau J, Yakar S, Brodt P
Molecular therapy : the journal of the American Society of Gene Therapy 2009 Jul;17(7):1241-9
Molecular therapy : the journal of the American Society of Gene Therapy 2009 Jul;17(7):1241-9
Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes.
Bäck K, Arnqvist HJ
Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society 2009 Apr;19(2):101-11
Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society 2009 Apr;19(2):101-11
Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foà R, Peschle C, Sporn M, Testa U
Leukemia research 2008 Aug;32(8):1244-58
Leukemia research 2008 Aug;32(8):1244-58
Insulin-like growth factor (IGF) 2 stimulates steroidogenesis and mitosis of bovine granulosa cells through the IGF1 receptor: role of follicle-stimulating hormone and IGF2 receptor.
Spicer LJ, Aad PY
Biology of reproduction 2007 Jul;77(1):18-27
Biology of reproduction 2007 Jul;77(1):18-27
Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foà R, Peschle C, Testa U
Stem cells (Dayton, Ohio) 2007 Aug;25(8):1862-71
Stem cells (Dayton, Ohio) 2007 Aug;25(8):1862-71
The susceptibility of granulosa cells to apoptosis is influenced by oestradiol and the cell cycle.
Quirk SM, Cowan RG, Harman RM
The Journal of endocrinology 2006 Jun;189(3):441-53
The Journal of endocrinology 2006 Jun;189(3):441-53
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IGF-I R/IGF1R by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, SK-Mel-28 human malignant melanoma cell line, and G361 human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGF-I R/IGF1R at approximately 275 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IGF-I R/IGF1R in MCF-7 and HDLM Human Cell Lines. IGF-I R/IGF1R was detected in immersion fixed MCF-7 human breast cancer cell line (positive staining; left panel) and HDLM human Hodgkin's lymphoma cell line (negative staining; right panel) using Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane in MCF-7 cell line. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IGF-I R/IGF1R in Mouse Heart. IGF-I R/IGF1R was detected in perfusion fixed paraffin-embedded sections of mouse heart using Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IGF-I R/IGF1R in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IGF-I and Neutralization by Human IGF-I R/IGF1R Antibody. Recombinant Human (rh) IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by rhIGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391). At 11 µg/mL, this antibody will neutralize 50-75% rhIGF-1 induced activity.
