Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ARG51590 - Provider product page
- Provider
- Arigo
- Product name
- anti-IGF-1R phospho (Tyr1161) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide sequence around phosphorylation site of tyrosine 1161(D-I-Y(p)-E-T)derived from Human IGF-1R.
- Description
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 293 and SKOV3 cells treated or un-treated with insulin stained with IGF-1R antibody (Line 1, 2 and 3) and IGF-1R (phospho-Tyr1161) antibody (ARG51590, Line 4 and 5). Line3 and Line4 also pre-incubated with the peptides used for antibody raising.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: methanol-fixed MCF7 cells stained with anti-IGF-1R (phospho Tyr1161) antibody ARG51590, Red).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin- embedded human breast carcinoma tissue stained with anti-IGF-1R (phospho Tyr1161) antibody ARG51590).