Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 53-8849-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD221 (IGF1R) Monoclonal Antibody (1H7), Alexa Fluor 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 1H7 monoclonal antibody reacts with human insulin-like growth factor-1 receptor (IGF-1R), also known as CD221. This receptor is a cell surface-expressed glycoprotein composed of two extracellular alpha subunits and two transmembrane beta subunits that possess tyrosine kinase activity. Expressed on nearly all cell types, this receptor binds IGF-I and IGF-II, as well as insulin. Ligand binding leads to activation of the PI3K/Akt and MAPK pathways, which mediate cell proliferation and survival. Many tumors and transformed cells display altered IGF-1R expression. This monoclonal antibody has been reported to block binding of IGF-I and IGF-II to the IGF-1R. Applications Reported: This 1H7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1H7 antibody has been pre-titrated and tested by flow cytometric analysis of the HeLa cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 1H7
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Wingless-type family member 3A triggers neuronal polarization via cross-activation of the insulin-like growth factor-1 receptor pathway.
Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor.
The insulin-like growth factor 1 receptor is required for the proliferation of hemopoietic cells.
Bernis ME, Oksdath M, Dupraz S, Nieto Guil A, Fernández MM, Malchiodi EL, Rosso SB, Quiroga S
Frontiers in cellular neuroscience 2013;7:194
Frontiers in cellular neuroscience 2013;7:194
Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor.
Li SL, Kato J, Paz IB, Kasuya J, Fujita-Yamaguchi Y
Biochemical and biophysical research communications 1993 Oct 15;196(1):92-8
Biochemical and biophysical research communications 1993 Oct 15;196(1):92-8
The insulin-like growth factor 1 receptor is required for the proliferation of hemopoietic cells.
Reiss K, Porcu P, Sell C, Pietrzkowski Z, Baserga R
Oncogene 1992 Nov;7(11):2243-8
Oncogene 1992 Nov;7(11):2243-8
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of HeLa cells with Mouse IgG1 K Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (blue histogram) or Anti-Human CD221 (Insulin-like Growth Factor-1 Receptor) Alexa Fluor® 488 (purple histogram). Total viable cells were used for analysis.
- Conjugate
- Green dye