53-8849-41

antibody from Invitrogen Antibodies
Targeting: IGF1R CD221, IGFIR, IGFR, JTK13, MGC18216
Provider product page for 53-8849-41

Antibody data

Product number
53-8849-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD221 (IGF1R) Monoclonal Antibody (1H7), Alexa Fluor 488, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This 1H7 monoclonal antibody reacts with human insulin-like growth factor-1 receptor (IGF-1R), also known as CD221. This receptor is a cell surface-expressed glycoprotein composed of two extracellular alpha subunits and two transmembrane beta subunits that possess tyrosine kinase activity. Expressed on nearly all cell types, this receptor binds IGF-I and IGF-II, as well as insulin. Ligand binding leads to activation of the PI3K/Akt and MAPK pathways, which mediate cell proliferation and survival. Many tumors and transformed cells display altered IGF-1R expression. This monoclonal antibody has been reported to block binding of IGF-I and IGF-II to the IGF-1R. Applications Reported: This 1H7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1H7 antibody has been pre-titrated and tested by flow cytometric analysis of the HeLa cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Green dye
Isotype
IgG
Antibody clone number
1H7
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
IGF1R protein structure - 53-8849-41 shown in red.