Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 53-8849-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD221 (IGF1R) Monoclonal Antibody (1H7), Alexa Fluor™ 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 1H7 monoclonal antibody reacts with human insulin-like growth factor-1 receptor (IGF-1R), also known as CD221. This receptor is a cell surface-expressed glycoprotein composed of two extracellular alpha subunits and two transmembrane beta subunits that possess tyrosine kinase activity. Expressed on nearly all cell types, this receptor binds IGF-I and IGF-II, as well as insulin. Ligand binding leads to activation of the PI3K/Akt and MAPK pathways, which mediate cell proliferation and survival. Many tumors and transformed cells display altered IGF-1R expression. This monoclonal antibody has been reported to block binding of IGF-I and IGF-II to the IGF-1R. Applications Reported: This 1H7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1H7 antibody has been pre-titrated and tested by flow cytometric analysis of the HeLa cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 1H7
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma.
Wingless-type family member 3A triggers neuronal polarization via cross-activation of the insulin-like growth factor-1 receptor pathway.
Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor.
The insulin-like growth factor 1 receptor is required for the proliferation of hemopoietic cells.
Ferreira Mendes JM, de Faro Valverde L, Torres Andion Vidal M, Paredes BD, Coelho P, Allahdadi KJ, Coletta RD, Souza BSF, Rocha CAG
International journal of molecular sciences 2020 Sep 5;21(18)
International journal of molecular sciences 2020 Sep 5;21(18)
Wingless-type family member 3A triggers neuronal polarization via cross-activation of the insulin-like growth factor-1 receptor pathway.
Bernis ME, Oksdath M, Dupraz S, Nieto Guil A, Fernández MM, Malchiodi EL, Rosso SB, Quiroga S
Frontiers in cellular neuroscience 2013;7:194
Frontiers in cellular neuroscience 2013;7:194
Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor.
Li SL, Kato J, Paz IB, Kasuya J, Fujita-Yamaguchi Y
Biochemical and biophysical research communications 1993 Oct 15;196(1):92-8
Biochemical and biophysical research communications 1993 Oct 15;196(1):92-8
The insulin-like growth factor 1 receptor is required for the proliferation of hemopoietic cells.
Reiss K, Porcu P, Sell C, Pietrzkowski Z, Baserga R
Oncogene 1992 Nov;7(11):2243-8
Oncogene 1992 Nov;7(11):2243-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of HeLa cells with Mouse IgG1 K Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (blue histogram) or Anti-Human CD221 (Insulin-like Growth Factor-1 Receptor) Alexa Fluor® 488 (purple histogram). Total viable cells were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Evaluation of IGF-1, IGF1R and HH pathway expression in fibroblasts and oral squamous carcinoma cells. ( A ) Representative histogram of IGF1R (CD221, dark histogram) expression in SCC-4 cells assessed by flow cytometry. Unstained cells were used as controls (lighter histogram). ( B ) IGF1 mRNA expression in SCC-4 and MF1 cells, evaluated by RT-qPCR. ( C ) Evaluation of HH pathway component expression in MF1 by immunocytochemistry. Magnification: 400x.
- Conjugate
- Green dye