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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-7177 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-7177, RRID:AB_2165556
- Product name
- Anti-PIK3CG
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Syk-mediated translocation of PI3Kdelta to the leading edge controls lamellipodium formation and migration of leukocytes.
Polycystin-1 induces resistance to apoptosis through the phosphatidylinositol 3-kinase/Akt signaling pathway.
PI3 kinase is important for Ras, MEK and Erk activation of Epo-stimulated human erythroid progenitors.
Schymeinsky J, Then C, Sindrilaru A, Gerstl R, Jakus Z, Tybulewicz VL, Scharffetter-Kochanek K, Walzog B
PloS one 2007 Nov 7;2(11):e1132
PloS one 2007 Nov 7;2(11):e1132
Polycystin-1 induces resistance to apoptosis through the phosphatidylinositol 3-kinase/Akt signaling pathway.
Boca M, Distefano G, Qian F, Bhunia AK, Germino GG, Boletta A
Journal of the American Society of Nephrology : JASN 2006 Mar;17(3):637-47
Journal of the American Society of Nephrology : JASN 2006 Mar;17(3):637-47
PI3 kinase is important for Ras, MEK and Erk activation of Epo-stimulated human erythroid progenitors.
Schmidt EK, Fichelson S, Feller SM
BMC biology 2004 May 18;2:7
BMC biology 2004 May 18;2:7
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 126
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.
- Validation comment
- No experimental and/or bioinformatic data available, or staining pattern not consistent with experimental and/or bioinformatic data.
