TA505228

antibody from OriGene

Targeting: PIK3CG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505228

Provider

OriGene

Proper citation

OriGene Cat#TA505228, RRID:AB_2622934

Product name

PIK3CG mouse monoclonal antibody,clone OTI6H5

Antibody type

Monoclonal

Description

PIK3CG mouse monoclonal antibody,clone OTI6H5

Host

Mouse

Conjugate

Unconjugated

Epitope

PIK3CG

Isotype

IgG

Antibody clone number

OTI6H5

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PIK3CG monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3CG (RC207790, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3CG.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PIK3CG mouse monoclonal antibody (TA505228) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIK3CG(RC207790).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PIK3CG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505228)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PIK3CG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505228)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PIK3CG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505228)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIK3CG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505228)

Validation comment

IHC