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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB4069 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4069, RRID:AB_1578768
- Product name
- PIK3CG polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PIK3CG.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Activation of the hexosamine pathway leads to phosphorylation of insulin receptor substrate-1 on Ser307 and Ser612 and impairs the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin insulin biosynthetic pathway in RIN pancreatic beta-cells.
Inhibition of the PI3K-Akt signaling pathway enhances the sensitivity of Fas-mediated apoptosis in human gastric carcinoma cell line, MKN-45.
The IL-1 receptor accessory protein is essential for PI 3-kinase recruitment and activation.
Andreozzi F, D'Alessandris C, Federici M, Laratta E, Del Guerra S, Del Prato S, Marchetti P, Lauro R, Perticone F, Sesti G
Endocrinology 2004 Jun;145(6):2845-57
Endocrinology 2004 Jun;145(6):2845-57
Inhibition of the PI3K-Akt signaling pathway enhances the sensitivity of Fas-mediated apoptosis in human gastric carcinoma cell line, MKN-45.
Osaki M, Kase S, Adachi K, Takeda A, Hashimoto K, Ito H
Journal of cancer research and clinical oncology 2004 Jan;130(1):8-14
Journal of cancer research and clinical oncology 2004 Jan;130(1):8-14
The IL-1 receptor accessory protein is essential for PI 3-kinase recruitment and activation.
Reddy SA, Lin YF, Huang HJ, Samanta AK, Liao WS
Biochemical and biophysical research communications 2004 Apr 16;316(4):1022-8
Biochemical and biophysical research communications 2004 Apr 16;316(4):1022-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3CG polyclonal antibody (Cat # PAB4069) in HeLa cell lysate (35 ug/lane). PIK3CG (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3CG polyclonal antibody (Cat # PAB4069) in 293 cell lysate transiently transfected with the PIK3CG gene (2 ug/lane). PIK3CG (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human muscle tissue reacted with PIK3CG polyclonal antibody (Cat # PAB4069) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
