Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- A01517-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-PI3K-gamma/PIK3CG Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for PI3K-gamma/PIK3CG detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Establishment of diabetes mellitus model using Bombyx mori silkworms in a low-temperature environment.
A comparative study of Mesenchymal Stem Cells transplantation approach to antagonize age-associated ovarian hypofunction with consideration of safety and efficiency.
Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway.
Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.
Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells.
RhoA/Rock2/Limk1/cofilin1 pathway is involved in attenuation of neuronal dendritic spine loss by paeonol in the frontal cortex of D-galactose and aluminum‑induced Alzheimer's disease‑like rat model.
Avβ3 single-stranded DNA aptamer attenuates vascular restenosis via Ras-PI3K/MAPK pathway in rats after percutaneous transluminal coronary angioplasty.
Optimized integration of fluoxetine and 7, 8-dihydroxyflavone as an efficient therapy for reversing depressive-like behavior in mice during the perimenopausal period.
Hou J, Tan C, Chen N, Zhou Y, Huang S, Chen H, Qian L
Archives of insect biochemistry and physiology 2024 Jan;115(1):e22083
Archives of insect biochemistry and physiology 2024 Jan;115(1):e22083
A comparative study of Mesenchymal Stem Cells transplantation approach to antagonize age-associated ovarian hypofunction with consideration of safety and efficiency.
Wang L, Mei Q, Xie Q, Li H, Su P, Zhang L, Li K, Ma D, Chen G, Li J, Xiang W
Journal of advanced research 2022 May;38:245-259
Journal of advanced research 2022 May;38:245-259
Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway.
Xu Y, Chen W, Chen Z, Huang M, Yang F, Zhang Y
Drug design, development and therapy 2021;15:753-767
Drug design, development and therapy 2021;15:753-767
Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.
Nie L, Liu M, Chen J, Wu Q, Li Y, Yi J, Zheng X, Zhang J, Chu C, Yang J
Molecular medicine reports 2021 Apr;23(4)
Molecular medicine reports 2021 Apr;23(4)
Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells.
Kong L, Wu Y, Hu W, Liu L, Xue Y, Liang G
Environmental pollution (Barking, Essex : 1987) 2021 Apr 15;275:116556
Environmental pollution (Barking, Essex : 1987) 2021 Apr 15;275:116556
RhoA/Rock2/Limk1/cofilin1 pathway is involved in attenuation of neuronal dendritic spine loss by paeonol in the frontal cortex of D-galactose and aluminum‑induced Alzheimer's disease‑like rat model.
Han F, Xu H, Shen JX, Pan C, Yu ZH, Chen JJ, Zhu XL, Cai YF, Lu YP
Acta neurobiologiae experimentalis 2020;80(3):225-244
Acta neurobiologiae experimentalis 2020;80(3):225-244
Avβ3 single-stranded DNA aptamer attenuates vascular restenosis via Ras-PI3K/MAPK pathway in rats after percutaneous transluminal coronary angioplasty.
Wu HB, Wang ZW, Shi F, Ren ZL, Li LC, Hu XP, Hu R, Li BW
Artificial organs 2020 Jun;44(6):611-619
Artificial organs 2020 Jun;44(6):611-619
Optimized integration of fluoxetine and 7, 8-dihydroxyflavone as an efficient therapy for reversing depressive-like behavior in mice during the perimenopausal period.
Amin N, Xie S, Tan X, Chen Y, Ren Q, Botchway BOA, Hu S, Ma Y, Hu Z, Fang M
Progress in neuro-psychopharmacology & biological psychiatry 2020 Jul 13;101:109939
Progress in neuro-psychopharmacology & biological psychiatry 2020 Jul 13;101:109939
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of PI3K-gamma/PIK3CG using anti-PI3K-gamma/PIK3CG antibody (A01517-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI3K-gamma/PIK3CG antigen affinity purified polyclonal antibody (Catalog # A01517-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI3K-gamma/PIK3CG at approximately 126KD. The expected band size for PI3K-gamma/PIK3CG is at 126KD.
- Additional image