PA5-28070

antibody from Invitrogen Antibodies

Targeting: PIK3CG

Antibody data

Submit

Validation data

Product number: PA5-28070 - Provider product page
Provider: Invitrogen Antibodies
Product name: PIK3CG Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: PIK3CG-transfected 293T, Neuro 2A, Raw264.7, C8D30, PC-12.
Concentration: 1 mg/mL

PIK3CG protein structure - PA5-28070 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PI3 kinase p110 gamma in non-transfected lysate 293T lysate (A) and transfected lysate (B). Samples were resolved on 7.5% SDS PAGE gel and probed using a PI3 kinase p110 gamma polyclonal antibody (Product # PA5-28070) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PI3K gamma in Various whole cell extracts (30 µg). Samples were separated by 5% SDS-PAGE and the membrane was probed with PI3K gamma, C-term Polyclonal antibody (Product # PA5-28070) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PIK3CG was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PIK3CG Polyclonal Antibody (Product # PA5-28070) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PIK3CG was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PIK3CG Polyclonal Antibody (Product # PA5-28070) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PIK3CG was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PIK3CG Polyclonal Antibody (Product # PA5-28070) at a dilution of 1:1000.