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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- MAB3585 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat RIPK1/RIP1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse and rat RIPK1/RIP1 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q13546- Isotype
- IgG
- Antibody clone number
- 334640
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Interaction between the BAG1S isoform and HSP70 mediates the stability of anti-apoptotic proteins and the survival of osteosarcoma cells expressing oncogenic MYC.
Leishmania braziliensis Subverts Necroptosis by Modulating RIPK3 Expression.
Necrostatin-1 protects C2C12 myotubes from CoCl2-induced hypoxia.
RIP3 dependent NLRP3 inflammasome activation is implicated in acute lung injury in mice.
MPP+ induces necrostatin-1- and ferrostatin-1-sensitive necrotic death of neuronal SH-SY5Y cells.
Hypoxia potentiates the cytotoxic effect of piperlongumine in pheochromocytoma models.
ESCRT-0 dysfunction compromises autophagic degradation of protein aggregates and facilitates ER stress-mediated neurodegeneration via apoptotic and necroptotic pathways.
Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.
TWEAK induces apoptosis through a death-signaling complex comprising receptor-interacting protein 1 (RIP1), Fas-associated death domain (FADD), and caspase-8.
Gennaro VJ, Wedegaertner H, McMahon SB
BMC cancer 2019 Mar 22;19(1):258
BMC cancer 2019 Mar 22;19(1):258
Leishmania braziliensis Subverts Necroptosis by Modulating RIPK3 Expression.
Luz NF, Khouri R, Van Weyenbergh J, Zanette DL, Fiuza PP, Noronha A, Barral A, Boaventura VS, Prates DB, Chan FK, Andrade BB, Borges VM
Frontiers in microbiology 2018;9:2283
Frontiers in microbiology 2018;9:2283
Necrostatin-1 protects C2C12 myotubes from CoCl2-induced hypoxia.
Chen R, Xu J, She Y, Jiang T, Zhou S, Shi H, Li C
International journal of molecular medicine 2018 May;41(5):2565-2572
International journal of molecular medicine 2018 May;41(5):2565-2572
RIP3 dependent NLRP3 inflammasome activation is implicated in acute lung injury in mice.
Chen J, Wang S, Fu R, Zhou M, Zhang T, Pan W, Yang N, Huang Y
Journal of translational medicine 2018 Aug 20;16(1):233
Journal of translational medicine 2018 Aug 20;16(1):233
MPP+ induces necrostatin-1- and ferrostatin-1-sensitive necrotic death of neuronal SH-SY5Y cells.
Ito K, Eguchi Y, Imagawa Y, Akai S, Mochizuki H, Tsujimoto Y
Cell death discovery 2017;3:17013
Cell death discovery 2017;3:17013
Hypoxia potentiates the cytotoxic effect of piperlongumine in pheochromocytoma models.
Bullova P, Cougnoux A, Abunimer L, Kopacek J, Pastorekova S, Pacak K
Oncotarget 2016 Jun 28;7(26):40531-40545
Oncotarget 2016 Jun 28;7(26):40531-40545
ESCRT-0 dysfunction compromises autophagic degradation of protein aggregates and facilitates ER stress-mediated neurodegeneration via apoptotic and necroptotic pathways.
Oshima R, Hasegawa T, Tamai K, Sugeno N, Yoshida S, Kobayashi J, Kikuchi A, Baba T, Futatsugi A, Sato I, Satoh K, Takeda A, Aoki M, Tanaka N
Scientific reports 2016 Apr 26;6:24997
Scientific reports 2016 Apr 26;6:24997
Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.
Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N
PloS one 2014;9(6):e100787
PloS one 2014;9(6):e100787
TWEAK induces apoptosis through a death-signaling complex comprising receptor-interacting protein 1 (RIP1), Fas-associated death domain (FADD), and caspase-8.
Ikner A, Ashkenazi A
The Journal of biological chemistry 2011 Jun 17;286(24):21546-54
The Journal of biological chemistry 2011 Jun 17;286(24):21546-54
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat RIPK1/RIP1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, DA3 mouse myeloma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RIPK1/RIP1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human RIPK1/RIP1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for RIPK1/RIP1 at approximately 78 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RIPK1/RIP1 in MCF-7 Human Cell Line. RIPK1/RIP1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat RIPK1/RIP1 Monoclonal Antibody (Catalog # MAB3585) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
