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- Antibody Data
- Antigen structure
- References [17]
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- Product number
- M600B - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-2 Monoclonal Antibody (B-G5), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M600B targets IL-2 in ELISA, and WB applications and shows reactivity with Human samples. The M600B immunogen is recombinant human IL-2. M600B detects IL-2 which has a predicted molecular weight of approximately 15 kDa. The M600B IL2 antibody (biotinylated conjugate of clone BG5) has successfully been paired as the detection antibody in a sandwich ELISA with polyclonal capture antibody P600. Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. Biotinylated antibody M600B (clone BG5) and antibody P600 have successfully been used in combination with recombinant IL2 protein SIL2 in ELISA applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- B-G5
- Vial size
- 250 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references CARbodies: Human Antibodies Against Cell Surface Tumor Antigens Selected From Repertoires Displayed on T Cell Chimeric Antigen Receptors.
Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.
Lymphocyte display: a novel antibody selection platform based on T cell activation.
Cell-mediated immune responses and protective efficacy against infection with Mycobacterium tuberculosis induced by Hsp65 and hIL-2 fusion protein in mice.
Growth and differentiation advantages of CD4+ OX40+ T cells from allogeneic hematopoietic stem cell transplantation recipients.
Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.
IL-2 absorption affects IFN-gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays.
Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.
Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.
CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.
CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.
Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.
Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.
Immune responses to hepatitis C and non-hepatitis C antigens in hepatitis C virus infected and HIV-1 coinfected patients.
Immune responses to hepatitis C and non-hepatitis C antigens in hepatitis C virus infected and HIV-1 coinfected patients.
Naturally developing memory T cell xenoreactivity to swine antigens in human peripheral blood lymphocytes.
Pretransplant frequency of donor-specific, IFN-gamma-producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes.
Alonso-Camino V, Sánchez-Martín D, Compte M, Nuñez-Prado N, Diaz RM, Vile R, Alvarez-Vallina L
Molecular therapy. Nucleic acids 2013 May 21;2(5):e93
Molecular therapy. Nucleic acids 2013 May 21;2(5):e93
Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.
Loewenbrueck KF, Tigno-Aranjuez JT, Boehm BO, Lehmann PV, Tary-Lehmann M
Neurobiology of aging 2010 Oct;31(10):1732-42
Neurobiology of aging 2010 Oct;31(10):1732-42
Lymphocyte display: a novel antibody selection platform based on T cell activation.
Alonso-Camino V, Sánchez-Martín D, Compte M, Sanz L, Alvarez-Vallina L
PloS one 2009 Sep 24;4(9):e7174
PloS one 2009 Sep 24;4(9):e7174
Cell-mediated immune responses and protective efficacy against infection with Mycobacterium tuberculosis induced by Hsp65 and hIL-2 fusion protein in mice.
Shi C, Yuan S, Zhang H, Zhang T, Wang L, Xu Z
Scandinavian journal of immunology 2009 Feb;69(2):140-9
Scandinavian journal of immunology 2009 Feb;69(2):140-9
Growth and differentiation advantages of CD4+ OX40+ T cells from allogeneic hematopoietic stem cell transplantation recipients.
Shindo T, Ishikawa T, Fukunaga A, Hori T, Uchiyama T
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2008 Mar;14(3):268-81
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2008 Mar;14(3):268-81
Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.
Nowacki TM, Kuerten S, Zhang W, Shive CL, Kreher CR, Boehm BO, Lehmann PV, Tary-Lehmann M
Cellular immunology 2007 May;247(1):36-48
Cellular immunology 2007 May;247(1):36-48
IL-2 absorption affects IFN-gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays.
Quast S, Zhang W, Shive C, Kovalovski D, Ott PA, Herzog BA, Boehm BO, Tary-Lehmann M, Karulin AY, Lehmann PV
Cellular immunology 2005 Sep;237(1):28-36
Cellular immunology 2005 Sep;237(1):28-36
Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.
Kuekrek H, Schlingmann T, Valdez H, Boehm BO, Pollard RB, Mitsuyasu R, Goebel FD, Lederman MM, Lehmann PV, Tary-Lehmann M
AIDS (London, England) 2005 Nov 18;19(17):1967-74
AIDS (London, England) 2005 Nov 18;19(17):1967-74
Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.
Kuekrek H, Schlingmann T, Valdez H, Boehm BO, Pollard RB, Mitsuyasu R, Goebel FD, Lederman MM, Lehmann PV, Tary-Lehmann M
AIDS (London, England) 2005 Nov 18;19(17):1967-74
AIDS (London, England) 2005 Nov 18;19(17):1967-74
CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.
Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M
Journal of immunological methods 2003 Jul;278(1-2):79-93
Journal of immunological methods 2003 Jul;278(1-2):79-93
CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.
Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M
Journal of immunological methods 2003 Jul;278(1-2):79-93
Journal of immunological methods 2003 Jul;278(1-2):79-93
Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.
Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, Lehmann PV, Schwander SK
Journal of immunological methods 2003 Aug;279(1-2):111-21
Journal of immunological methods 2003 Aug;279(1-2):111-21
Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.
Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, Lehmann PV, Schwander SK
Journal of immunological methods 2003 Aug;279(1-2):111-21
Journal of immunological methods 2003 Aug;279(1-2):111-21
Immune responses to hepatitis C and non-hepatitis C antigens in hepatitis C virus infected and HIV-1 coinfected patients.
Valdez H, Anthony D, Farukhi F, Patki A, Salkowitz J, Heeger P, Peterson DL, Post AB, Asaad R, Lederman MM
AIDS (London, England) 2000 Oct 20;14(15):2239-46
AIDS (London, England) 2000 Oct 20;14(15):2239-46
Immune responses to hepatitis C and non-hepatitis C antigens in hepatitis C virus infected and HIV-1 coinfected patients.
Valdez H, Anthony D, Farukhi F, Patki A, Salkowitz J, Heeger P, Peterson DL, Post AB, Asaad R, Lederman MM
AIDS (London, England) 2000 Oct 20;14(15):2239-46
AIDS (London, England) 2000 Oct 20;14(15):2239-46
Naturally developing memory T cell xenoreactivity to swine antigens in human peripheral blood lymphocytes.
Hartig CV, Haller GW, Sachs DH, Kuhlenschmidt S, Heeger PS
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2790-6
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2790-6
Pretransplant frequency of donor-specific, IFN-gamma-producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes.
Heeger PS, Greenspan NS, Kuhlenschmidt S, Dejelo C, Hricik DE, Schulak JA, Tary-Lehmann M
Journal of immunology (Baltimore, Md. : 1950) 1999 Aug 15;163(4):2267-75
Journal of immunology (Baltimore, Md. : 1950) 1999 Aug 15;163(4):2267-75
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