Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1890 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects feline IL-2 in ELISAs and Western blots. In sandwich immunoassays, approximately 3% cross-reactivity with recombinant canine IL-2 is observed, approximately 1% cross reactivity with recombinant human IL-2 and recombinant porcine IL-2 is observed, and less than 0.2% cross-reactivity with recombinant mouse IL-2, recombinant equine IL-2, recombinant bovine IL-2, recombinant rat IL-2, and recombinant cotton rat IL-2 is observed.
- Reactivity
- Feline
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Vaccination with vif-deleted feline immunodeficiency virus provirus, GM-CSF, and TNF-alpha plasmids preserves global CD4 T lymphocyte function after challenge with FIV.
Maksaereekul S, Dubie RA, Shen X, Kieu H, Dean GA, Sparger EE
Vaccine 2009 Jun 8;27(28):3754-65
Vaccine 2009 Jun 8;27(28):3754-65
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-2 and Neutralization by Feline IL-2 Antibody. Recombinant Feline IL-2 (Cys146Ser) (Catalog # 1890-FL) induces cell proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Feline IL-2 (Cys146Ser) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890). The ND50 is typically 0.03-0.15 µg/mL.