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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- BAF1890 - Provider product page
- Provider
- R&D Systems
- Product name
- Feline IL-2 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects feline IL-2 in ELISAs and Western blots. In sandwich immunoassays, approximately 3% cross-reactivity with recombinant canine IL-2 is observed, approximately 1% cross-reactivity with recombinant porcine IL-2 and recombinant human IL-2 is observed, and less than 0.2% cross-reactivity with recombinant mouse IL-2, recombinant equine IL-2, recombinant bovine IL-2, recombinant rat IL-2, and recombinant cotton rat IL-2 is observed.
- Reactivity
- Feline
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
Q07885- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-2 in Feline PBMCs. IL-2 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline IL-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1890) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
