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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- MAB602-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Primate IL-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and primate IL-2 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-1 alpha , rhIL-1 beta , rhIL-3, rhIL-4, rhIL-6, rhIL-7, rhIL-8, recombinant mouse (rm) IL-1 beta , rmIL-2, rmIL-3, rmIL-4, rmIL-5, rmIL-6, or rmIL-7 is observed.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_000577- Isotype
- IgG
- Antibody clone number
- 5355
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Heterogeneous GM-CSF signaling in macrophages is associated with control of Mycobacterium tuberculosis.
IL2/Anti-IL2 Complex Combined with CTLA-4, But Not PD-1, Blockade Rescues Antitumor NK Cell Function by Regulatory T-cell Modulation.
Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology.
Key Residues at Third CDR3β Position Impact Structure and Antigen Recognition of Human Invariant NK TCRs.
Functional characterization of human T cell hyporesponsiveness induced by CTLA4-Ig.
Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Th2 cytokine response in Major Depressive Disorder patients before treatment.
Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
Bryson BD, Rosebrock TR, Tafesse FG, Itoh CY, Nibasumba A, Babunovic GH, Corleis B, Martin C, Keegan C, Andrade P, Realegeno S, Kwon D, Modlin RL, Fortune SM
Nature communications 2019 May 27;10(1):2329
Nature communications 2019 May 27;10(1):2329
IL2/Anti-IL2 Complex Combined with CTLA-4, But Not PD-1, Blockade Rescues Antitumor NK Cell Function by Regulatory T-cell Modulation.
Caudana P, Núñez NG, De La Rochere P, Pinto A, Denizeau J, Alonso R, Niborski LL, Lantz O, Sedlik C, Piaggio E
Cancer immunology research 2019 Mar;7(3):443-457
Cancer immunology research 2019 Mar;7(3):443-457
Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology.
Whitfield SJC, Taylor C, Risdall JE, Griffiths GD, Jones JTA, Williamson ED, Rijpkema S, Saraiva L, Vessillier S, Green AC, Carter AJ
Journal of immunology (Baltimore, Md. : 1950) 2017 May 15;198(10):3989-3998
Journal of immunology (Baltimore, Md. : 1950) 2017 May 15;198(10):3989-3998
Key Residues at Third CDR3β Position Impact Structure and Antigen Recognition of Human Invariant NK TCRs.
Chamoto K, Guo T, Scally SW, Kagoya Y, Anczurowski M, Wang CH, Rahman MA, Saso K, Butler MO, Chiu PP, Julien JP, Hirano N
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 1;198(3):1056-1065
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 1;198(3):1056-1065
Functional characterization of human T cell hyporesponsiveness induced by CTLA4-Ig.
Rochman Y, Yukawa M, Kartashov AV, Barski A
PloS one 2015;10(4):e0122198
PloS one 2015;10(4):e0122198
Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'.
Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC
Nature 2012 Mar 25;484(7395):529-33
Nature 2012 Mar 25;484(7395):529-33
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS (London, England) 2007 Jun 19;21(10):1263-72
AIDS (London, England) 2007 Jun 19;21(10):1263-72
Th2 cytokine response in Major Depressive Disorder patients before treatment.
Pavón L, Sandoval-López G, Eugenia Hernández M, Loría F, Estrada I, Pérez M, Moreno J, Avila U, Leff P, Antón B, Heinze G
Journal of neuroimmunology 2006 Mar;172(1-2):156-65
Journal of neuroimmunology 2006 Mar;172(1-2):156-65
Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL
Journal of immunology (Baltimore, Md. : 1950) 2000 May 15;164(10):5337-43
Journal of immunology (Baltimore, Md. : 1950) 2000 May 15;164(10):5337-43
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-2 in Human PBMCs. IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Mouse Anti-Human/Primate IL-2 Monoclonal Antibody (Catalog # MAB602) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
