Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB702-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-2 in ELISAs.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- JES6-1A12
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC.
Interleukin-7 optimizes FOXP3+CD4+ regulatory T cells reactivity to interleukin-2 by modulating CD25 expression.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
John B, Naczki C, Patel C, Ghoneum A, Qasem S, Salih Z, Said N
Oncogene 2019 May;38(22):4366-4383
Oncogene 2019 May;38(22):4366-4383
Interleukin-7 optimizes FOXP3+CD4+ regulatory T cells reactivity to interleukin-2 by modulating CD25 expression.
Simonetta F, Gestermann N, Bloquet S, Bourgeois C
PloS one 2014;9(12):e113314
PloS one 2014;9(12):e113314
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):409-19
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):409-19
Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2130-9
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2130-9
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
Blood 2008 Jul 15;112(2):362-73
Blood 2008 Jul 15;112(2):362-73
Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
Bednarski JJ, Warner RE, Rao T, Leonetti F, Yung R, Richardson BC, Johnson KJ, Ellman JA, Opipari AW Jr, Glick GD
Arthritis and rheumatism 2003 Mar;48(3):757-66
Arthritis and rheumatism 2003 Mar;48(3):757-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-2 and Neutralization by Mouse IL-2 Antibody. Recombinant Mouse IL-2 (Catalog # 402-ML) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-2 Monoclonal Antibody (Catalog # MAB702). The ND50 is typically 0.125-0.75 µg/mL.