Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB202-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_000577
- Isotype
- IgG
- Antibody clone number
- 5334
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Effects of pepsin and pepstatin on reflux tonsil hypertrophy in vitro.
Blocking IL-2 Signal In Vivo with an IL-2 Antagonist Reduces Tumor Growth through the Control of Regulatory T Cells.
IL-23 and IL-1β Drive Human Th17 Cell Differentiation and Metabolic Reprogramming in Absence of CD28 Costimulation.
Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling.
Activin A programs the differentiation of human TFH cells.
Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
Interleukin-2 alters distribution of CD144 (VE-cadherin) in endothelial cells.
ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive.
T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue.
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Reprogramming of IL-10 activity and signaling by IFN-gamma.
Kim JH, Jang SJ, Yun JW, Jung MH, Woo SH
PloS one 2018;13(11):e0207090
PloS one 2018;13(11):e0207090
Blocking IL-2 Signal In Vivo with an IL-2 Antagonist Reduces Tumor Growth through the Control of Regulatory T Cells.
Carmenate T, Ortíz Y, Enamorado M, García-Martínez K, Avellanet J, Moreno E, Graça L, León K
Journal of immunology (Baltimore, Md. : 1950) 2018 May 15;200(10):3475-3484
Journal of immunology (Baltimore, Md. : 1950) 2018 May 15;200(10):3475-3484
IL-23 and IL-1β Drive Human Th17 Cell Differentiation and Metabolic Reprogramming in Absence of CD28 Costimulation.
Revu S, Wu J, Henkel M, Rittenhouse N, Menk A, Delgoffe GM, Poholek AC, McGeachy MJ
Cell reports 2018 Mar 6;22(10):2642-2653
Cell reports 2018 Mar 6;22(10):2642-2653
Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling.
Jiang J, Chen X, An H, Yang B, Zhang F, Cheng X
Scientific reports 2016 Sep 2;6:32320
Scientific reports 2016 Sep 2;6:32320
Activin A programs the differentiation of human TFH cells.
Locci M, Wu JE, Arumemi F, Mikulski Z, Dahlberg C, Miller AT, Crotty S
Nature immunology 2016 Aug;17(8):976-84
Nature immunology 2016 Aug;17(8):976-84
Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
The Journal of biological chemistry 2015 Sep 11;290(37):22338-51
Interleukin-2 alters distribution of CD144 (VE-cadherin) in endothelial cells.
Kim DW, Zloza A, Broucek J, Schenkel JM, Ruby C, Samaha G, Kaufman HL
Journal of translational medicine 2014 May 6;12:113
Journal of translational medicine 2014 May 6;12:113
ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
Weissmüller S, Semmler LY, Kalinke U, Christians S, Müller-Berghaus J, Waibler Z
Blood 2012 Jun 28;119(26):6268-77
Blood 2012 Jun 28;119(26):6268-77
Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive.
Hutnick NA, Carnathan D, Demers K, Makedonas G, Ertl HC, Betts MR
Vaccine 2010 Feb 23;28(8):1932-41
Vaccine 2010 Feb 23;28(8):1932-41
T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
Blood 2007 Jun 1;109(11):4856-64
Blood 2007 Jun 1;109(11):4856-64
Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue.
Karenko L, Hahtola S, Päivinen S, Karhu R, Syrjä S, Kähkönen M, Nedoszytko B, Kytölä S, Zhou Y, Blazevic V, Pesonen M, Nevala H, Nupponen N, Sihto H, Krebs I, Poustka A, Roszkiewicz J, Saksela K, Peterson P, Visakorpi T, Ranki A
Cancer research 2005 Sep 15;65(18):8101-10
Cancer research 2005 Sep 15;65(18):8101-10
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
Immunology 2004 Jun;112(2):274-89
Immunology 2004 Jun;112(2):274-89
Reprogramming of IL-10 activity and signaling by IFN-gamma.
Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5034-41
Journal of immunology (Baltimore, Md. : 1950) 2003 Nov 15;171(10):5034-41
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-2 in Human PBMCs. IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-2 and Neutralization by Human IL-2 Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202). The ND50 is typically 0.015-0.03 µg/mL.