Antibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [9]
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- Product number
- 14-7029-85 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-2 Monoclonal Antibody (MQ1-17H12), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.
- Antibody clone number
- MQ1-17H12
- Concentration
- 0.5 mg/mL
Submitted references Dynamics of spike-and nucleocapsid specific immunity during long-term follow-up and vaccination of SARS-CoV-2 convalescents.
Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.
Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV.
Platelets stimulate programmed death-ligand 1 expression by cancer cells: Inhibition by anti-platelet drugs.
Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.
NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca(2+) entry in T cells.
A novel format for recombinant antibody-interleukin-2 fusion proteins exhibits superior tumor-targeting properties in vivo.
A Novel Fully-Human Potency-Matched Dual Cytokine-Antibody Fusion Protein Targets Carbonic Anhydrase IX in Renal Cell Carcinomas.
Antibody-Based Delivery of Cytokine Payloads to Carbonic Anhydrase IX Leads to Cancer Cures in Immunocompetent Tumor-Bearing Mice.
TIGIT and PD-1 Mark Intratumoral T Cells with Reduced Effector Function in B-cell Non-Hodgkin Lymphoma.
A novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignancies.
Optimization of mass cytometry sample cryopreservation after staining.
Prostate Cancer Cells Express More Androgen Receptor (AR) Following Androgen Deprivation, Improving Recognition by AR-Specific T Cells.
Loss of immune tolerance to IL-2 in type 1 diabetes.
Effects of the levonorgestrel-releasing intrauterine device on the immune microenvironment of the human cervix and endometrium.
PD-1 marks dysfunctional regulatory T cells in malignant gliomas.
Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.
Fiber composite slices for multiplexed immunoassays.
Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.
A vaccine against CCR5 protects a subset of macaques upon intravaginal challenge with simian immunodeficiency virus SIVmac251.
CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques.
Concomitant in vivo production of 19 different cytokines in human tonsils.
Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Koerber N, Priller A, Yazici S, Bauer T, Cheng CC, Mijočević H, Wintersteller H, Jeske S, Vogel E, Feuerherd M, Tinnefeld K, Winter C, Ruland J, Gerhard M, Haller B, Christa C, Zelger O, Roggendorf H, Halle M, Erber J, Lingor P, Keppler O, Zehn D, Protzer U, Knolle PA
Nature communications 2022 Jan 10;13(1):153
Nature communications 2022 Jan 10;13(1):153
Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.
Kathamuthu GR, Kumar NP, Moideen K, Menon PA, Babu S
Frontiers in cellular and infection microbiology 2021;11:756854
Frontiers in cellular and infection microbiology 2021;11:756854
Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV.
Alrubayyi A, Gea-Mallorquí E, Touizer E, Hameiri-Bowen D, Kopycinski J, Charlton B, Fisher-Pearson N, Muir L, Rosa A, Roustan C, Earl C, Cherepanov P, Pellegrino P, Waters L, Burns F, Kinloch S, Dong T, Dorrell L, Rowland-Jones S, McCoy LE, Peppa D
Nature communications 2021 Oct 5;12(1):5839
Nature communications 2021 Oct 5;12(1):5839
Platelets stimulate programmed death-ligand 1 expression by cancer cells: Inhibition by anti-platelet drugs.
Asgari A, Lesyk G, Poitras E, Govindasamy N, Terry K, To R, Back V, Rudzinski JK, Lewis JD, Jurasz P
Journal of thrombosis and haemostasis : JTH 2021 Nov;19(11):2862-2872
Journal of thrombosis and haemostasis : JTH 2021 Nov;19(11):2862-2872
Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.
Schwabenland M, Salié H, Tanevski J, Killmer S, Lago MS, Schlaak AE, Mayer L, Matschke J, Püschel K, Fitzek A, Ondruschka B, Mei HE, Boettler T, Neumann-Haefelin C, Hofmann M, Breithaupt A, Genc N, Stadelmann C, Saez-Rodriguez J, Bronsert P, Knobeloch KP, Blank T, Thimme R, Glatzel M, Prinz M, Bengsch B
Immunity 2021 Jul 13;54(7):1594-1610.e11
Immunity 2021 Jul 13;54(7):1594-1610.e11
NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca(2+) entry in T cells.
Wu B, Woo JS, Vila P, Jew M, Leung J, Sun Z, Srikanth S, Gwack Y
Cell reports 2021 Aug 24;36(8):109603
Cell reports 2021 Aug 24;36(8):109603
A novel format for recombinant antibody-interleukin-2 fusion proteins exhibits superior tumor-targeting properties in vivo.
Ongaro T, Gouyou B, Stringhini M, Corbellari R, Neri D, Villa A
Oncotarget 2020 Oct 13;11(41):3698-3711
Oncotarget 2020 Oct 13;11(41):3698-3711
A Novel Fully-Human Potency-Matched Dual Cytokine-Antibody Fusion Protein Targets Carbonic Anhydrase IX in Renal Cell Carcinomas.
De Luca R, Gouyou B, Ongaro T, Villa A, Ziffels B, Sannino A, Buttinoni G, Galeazzi S, Mazzacuva M, Neri D
Frontiers in oncology 2019;9:1228
Frontiers in oncology 2019;9:1228
Antibody-Based Delivery of Cytokine Payloads to Carbonic Anhydrase IX Leads to Cancer Cures in Immunocompetent Tumor-Bearing Mice.
Ziffels B, Stringhini M, Probst P, Fugmann T, Sturm T, Neri D
Molecular cancer therapeutics 2019 Sep;18(9):1544-1554
Molecular cancer therapeutics 2019 Sep;18(9):1544-1554
TIGIT and PD-1 Mark Intratumoral T Cells with Reduced Effector Function in B-cell Non-Hodgkin Lymphoma.
Josefsson SE, Beiske K, Blaker YN, Førsund MS, Holte H, Østenstad B, Kimby E, Köksal H, Wälchli S, Bai B, Smeland EB, Levy R, Kolstad A, Huse K, Myklebust JH
Cancer immunology research 2019 Mar;7(3):355-362
Cancer immunology research 2019 Mar;7(3):355-362
A novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignancies.
De Luca R, Kachel P, Kropivsek K, Snijder B, Manz MG, Neri D
Protein engineering, design & selection : PEDS 2018 May 1;31(5):173-179
Protein engineering, design & selection : PEDS 2018 May 1;31(5):173-179
Optimization of mass cytometry sample cryopreservation after staining.
Sumatoh HR, Teng KW, Cheng Y, Newell EW
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2017 Jan;91(1):48-61
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2017 Jan;91(1):48-61
Prostate Cancer Cells Express More Androgen Receptor (AR) Following Androgen Deprivation, Improving Recognition by AR-Specific T Cells.
Olson BM, Gamat M, Seliski J, Sawicki T, Jeffery J, Ellis L, Drake CG, Weichert J, McNeel DG
Cancer immunology research 2017 Dec;5(12):1074-1085
Cancer immunology research 2017 Dec;5(12):1074-1085
Loss of immune tolerance to IL-2 in type 1 diabetes.
Pérol L, Lindner JM, Caudana P, Nunez NG, Baeyens A, Valle A, Sedlik C, Loirat D, Boyer O, Créange A, Cohen JL, Rogner UC, Yamanouchi J, Marchant M, Leber XC, Scharenberg M, Gagnerault MC, Mallone R, Battaglia M, Santamaria P, Hartemann A, Traggiai E, Piaggio E
Nature communications 2016 Oct 6;7:13027
Nature communications 2016 Oct 6;7:13027
Effects of the levonorgestrel-releasing intrauterine device on the immune microenvironment of the human cervix and endometrium.
Shanmugasundaram U, Hilton JF, Critchfield JW, Greenblatt RM, Giudice LC, Averbach S, Seidman D, Shacklett BL, Smith-McCune K
American journal of reproductive immunology (New York, N.Y. : 1989) 2016 Aug;76(2):137-48
American journal of reproductive immunology (New York, N.Y. : 1989) 2016 Aug;76(2):137-48
PD-1 marks dysfunctional regulatory T cells in malignant gliomas.
Lowther DE, Goods BA, Lucca LE, Lerner BA, Raddassi K, van Dijk D, Hernandez AL, Duan X, Gunel M, Coric V, Krishnaswamy S, Love JC, Hafler DA
JCI insight 2016 Apr 21;1(5)
JCI insight 2016 Apr 21;1(5)
Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.
Van Eyck L, De Somer L, Pombal D, Bornschein S, Frans G, Humblet-Baron S, Moens L, de Zegher F, Bossuyt X, Wouters C, Liston A
Arthritis & rheumatology (Hoboken, N.J.) 2015 Jun;67(6):1592-7
Arthritis & rheumatology (Hoboken, N.J.) 2015 Jun;67(6):1592-7
Fiber composite slices for multiplexed immunoassays.
Kim J, Bae S, Song S, Chung K, Kwon S
Biomicrofluidics 2015 Jul;9(4):044109
Biomicrofluidics 2015 Jul;9(4):044109
Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.
Arlehamn CL, Seumois G, Gerasimova A, Huang C, Fu Z, Yue X, Sette A, Vijayanand P, Peters B
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 15;193(6):2931-40
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 15;193(6):2931-40
A vaccine against CCR5 protects a subset of macaques upon intravaginal challenge with simian immunodeficiency virus SIVmac251.
Van Rompay KK, Hunter Z, Jayashankar K, Peabody J, Montefiori D, LaBranche CC, Keele BF, Jensen K, Abel K, Chackerian B
Journal of virology 2014 Feb;88(4):2011-24
Journal of virology 2014 Feb;88(4):2011-24
CD4 T cell depletion exacerbates acute Mycobacterium tuberculosis while reactivation of latent infection is dependent on severity of tissue depletion in cynomolgus macaques.
Lin PL, Rutledge T, Green AM, Bigbee M, Fuhrman C, Klein E, Flynn JL
AIDS research and human retroviruses 2012 Dec;28(12):1693-702
AIDS research and human retroviruses 2012 Dec;28(12):1693-702
Concomitant in vivo production of 19 different cytokines in human tonsils.
Andersson J, Abrams J, Björk L, Funa K, Litton M, Agren K, Andersson U
Immunology 1994 Sep;83(1):16-24
Immunology 1994 Sep;83(1):16-24
Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE
Immunological reviews 1992 Jun;127:5-24
Immunological reviews 1992 Jun;127:5-24
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Supportive validation
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- Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) (right). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD4 Monoclonal Antibody, APC (Product # 17-0049-42) and 0.125 µg IL-2 Monoclonal Antibody followed by IgG2a Monoclonal Antibody, PE (Product # 12-4817-82). Cells in the lymphocyte gate were used for analysis.
Supportive validation
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- Figure 1. NKD2 is involved in regulation of the Ca 2+ -NFAT signaling pathway in T cells (A) Representative immunoblot showing expression of NKD2 in lysates from HeLa cells, unstimulated Jurkat T cells, or those stimulated with PMA + ionomycin or anti-CD3 antibody (Ab) for 6 h. beta-Actin, loading control. Data are representative of two independent experiments. (B) Representative immunoblot showing expression of NKD2 in lysates of control or NKD2 knockout (KO) Jurkat T cells generated using two independent sgRNAs (#1 and #3). beta-Actin, loading control. Data are representative of two independent experiments. (C) Representative traces showing averaged SOCE from control (69 cells), NKD2 KO (KO sgRNA #1, 70 cells) Jurkat T cells, or NKD2 KO Jurkat T cells reconstituted for expression of NKD2 (KO sgRNA #1 + NKD2, 68 cells) after TCR stimulation using anti-CD3 antibodies, followed by ionomycin treatment in the presence of external solution containing 2 mM Ca 2+ . Bar graph (right) shows averaged baseline-subtracted SOCE (+-SEM) from three independent experiments with NKD2 KO cells generated using two independent sgRNAs (#1 and #3). (D) Representative immunoblot of NFATc2 levels in nuclear extracts from wild-type (WT) and NKD2 KO (sgRNA #1) Jurkat T cells stimulated with 1 muM ionomycin for indicated times. A nuclear protein, Ku86, was used as a loading control. Bar graph shows the densitometric analysis of relative band intensities of nuclear NFATc2. Data show means +- SEM of poo
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- Figure 6. NKD2-mediated ORAI1 + vesicle trafficking is crucial for the effector function of primary T cells (A) Representative histograms showing levels of NKD2 protein in primary T cells transduced with lentiviral vectors encoding scrambled sgRNA (control), NKD2 sgRNA #1, and NKD2 sgRNA #3, together with those encoding Cas9. Cells were permeabilized and stained with anti-NKD2 Ab. The bar graph (right) shows average (+-SEM) from four independent experiments. (B) Representative traces showing averaged SOCE from primary control (73 cells) and NKD2 KO (KO sgRNA #1, 82 cells; KO sgRNA #3, 71 cells) T cells after TCR stimulation using anti-CD3 Abs, followed by ionomycin treatment in the presence of external solution containing 2 mM Ca 2+ . Bar graph (right) shows averaged baseline-subtracted SOCE (+-SEM) from three independent experiments. (C) Representative histograms showing levels of newly inserted PM-resident ORAI1 protein in primary control and NKD2 KO effector T cells after TCR stimulation. Cells were transduced as described above to induce deletion of NKD2 and stimulated and stained as described in Figure 3B for detection of newly inserted PM-resident ORAI1. Bar graph (right) shows average (+-SEM) of pooled technical replicates from three independent donors. (D) Representative flow plots showing expression of IFN-gamma, IL-2, and TNF in control, NKD2 KO (sgRNA #1), and NKD2 KO (sgRNA #3) cells after re-stimulation with anti-CD3 and anti-CD28 Abs. Bar graph shows means +- SE
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- Fig. 5 Rapid induction of polyfunctional IL-2-secreting T cells in convalescents and naive individuals after BNT162b2 mRNA vaccination. ( a ) frequencies of spike-reactive cytokine-secreting cells in convalescents (red, n = 50 (m11), n = 54 (vacc), n = 23 (boost)), and naive individuals (blue, n = 39 (m11), n = 49 (vacc), n = 40 (boost)) at month 11 after SARS-CoV-2 infection, two weeks after BNT162b2 mRNA prime vaccination (vacc) and 2 weeks after boost vaccination (boost). b Heatmap revealing frequencies of individuals bearing S1-reactive mono- or polyfunctional cytokine-secreting cells; convalescents (c), naive individuals (n). c Median and standard deviation for S1-reactive cytokine-secreting cells. d , e S1-reactive IL-2 and IFNgamma-producing CD4 and CD8 T cells in convalescents (red, n = 19) and naive individuals (blue, n = 31) determined directly ex vivo by flow cytometry using intracellular cytokine staining. Statistical analyses by ANOVA, two-sided Mann-Whitney and two-sided Wilcoxon signed-rank tests ( a , e ). n.s. denotes not significant; * p < 0.05; ** p < 0.01; **** p < 0.0001.
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- 5 FIGURE Platelets suppress T-cell activation by A549 cell an effect reversed by eptifibatide. (A) Representative flow cytometry dot plots and (B) summary data demonstrating IL-2 expression in Jurkat cells after 6 h of co-culture with A549 cells that were pre-treated with/without platelets and eptifibitide (10 muM). N = 7. *, p -value
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- Fig. 4 Composition of SARS-CoV-2-specific T cells in convalescent HIV-negative and HIV-positive individuals. Intracellular cytokine staining (ICS) was performed to detect cytokine-producing T cells to the indicated peptide pools in HIV-negative (HIV-, n = 12) and HIV-positive individuals (HIV+, n = 11). a Representative flow cytometric plots for the identification of antigen-specific CD4 T cells based on double expression (CD154 + IFN-gamma + , CD154 + IL-2 + , and CD154 + TNF-alpha + ) following 6-h stimulation with media alone (control) or overlapping SARS-CoV-2 peptides against Spike pool 1 and 2 (Spike), Nucleoprotein (N), and Membrane protein (M) directly ex vivo. b Frequency of aggregated CD4 T-cell responses (CD154 + IFN-gamma + , CD154 + IL-2 + , and CD154 + TNF-alpha + ) against Spike, M/N or combined (Spike and M/N) peptide pools (HIV-, n = 12; HIV+, n = 11). Error bars represent SEM. c Pie charts representing the relative proportions of Spike, M/N, or total (combined Spike and M/N) CD4 T-cell responses for one (gray), two (green) or three (dark blue) cytokines, and pie arcs denoting IFN-gamma, TNF-alpha and IL-2. d Representative flow cytometric plots for the identification of antigen-specific CD8 T cells based on the expression of (IFN-gamma + , TNF-alpha + , and IL-2 + ) against the specified peptide pools or media alone (control). e Proportion of aggregated CD8 T-cell responses against Spike, M/N or combined (Spike and M/N) responses in HIV- ( n = 12) and HIV+ (
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- Figure 1 Decreased frequencies of gammadelta T cells expressing Th1 cytokines in LTB comorbidities. Peripheral blood mononuclear cells (PBMCs) were either untreated or treated with Mtb or positive control antigens for 18 h. The absolute (unstimulated, UNS) and antigen-stimulated (PPD, WCL, P/I) net frequencies of Th1 (IFNgamma, IL-2, TNFalpha) cytokines were shown in LTB DM (n = 20), LTB PDM (n = 20), and LTB NDM (n = 20) groups. Geometric mean values were represented using bars, and every circle denotes a single individual. Kruskal-Wallis test with multiple Dunn's comparison was used to determine the p values.