16-7027-38

antibody from Invitrogen Antibodies
Targeting: IL2 IL-2, TCGF
Provider product page for 16-7027-38
Blocking/Neutralizing
Other assay

Antibody data

Product number
16-7027-38 - Provider product page
Provider
Invitrogen Antibodies
Product name
IL-2 Monoclonal Antibody (AB12-3G4), Functional Grade, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This AB12-3G4 monoclonal antibody reacts with and inhibits the bioactivity of human IL-2. IL-2 is a 15 kDa cytokine expressed by B cells, T cells, and dendritic cells. IL-2 signals via a heterotrimeric receptor (CD25) and results in the activation of Jak/STAT pathways. IL-2 plays a critical role in the proliferation, activation, and differentiation of T cells. The development of CD4+CD25+ regulatory T cells is especially dependent on the presence of IL-2, making it an important immunoregulatory cytokine. Applications Reported: This AB12-3G4 monoclonal antibody inhibits the bioactivity of human IL-2. Applications Tested: The ND50 of AB12-3G4, as determined by the neutralization of Human IL-2-induced proliferation of the CTLL-2 cell line, is less than or equal to 60 ng/mL in the presence of 10 ng/mL Human IL-2 Recombinant Protein. The neutralization dose of this antibody will vary depending on cell type, assay method, and cytokine concentration. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
AB12-3G4
Vial size
5 mg
Concentration
1 mg/mL
Storage
4° C
IL2 protein structure - 16-7027-38 shown in red.