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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- AB-202-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-2 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human IL-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant porcine IL-2, recombinant mouse IL-2 and recombinant rat IL-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P60568- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-2 by Western Blot. Western blot shows lysates of monensin treated human peripheral blood mononuclear cells (PBMCs) with no additioan treatment (-) or additionaly treated (+) with 0.5 ug/mL calcium ionomycin (Iono) and 50 ng/mL PMA overnight. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human IL-2 Polyclonal Antibody (Catalog # AB-202-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-2 and Neutralization by Human IL-2 Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-2 Polyclonal Antibody (Catalog # AB-202-NA). The ND50 is typically 0.2-0.8 µg/mL.
