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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Immunocytochemistry [2]
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- Product number
- BAF202 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Primate IL-2 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and primate IL-2 in ELISAs and Western blots.
- Reactivity
- Human, Simian
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P60568- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Interference of the T Cell and Antigen-Presenting Cell Costimulatory Pathway Using CTLA4-Ig (Abatacept) Prevents Staphylococcal Enterotoxin B Pathology.
Key Residues at Third CDR3β Position Impact Structure and Antigen Recognition of Human Invariant NK TCRs.
Functional characterization of human T cell hyporesponsiveness induced by CTLA4-Ig.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Th2 cytokine response in Major Depressive Disorder patients before treatment.
Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
Whitfield SJC, Taylor C, Risdall JE, Griffiths GD, Jones JTA, Williamson ED, Rijpkema S, Saraiva L, Vessillier S, Green AC, Carter AJ
Journal of immunology (Baltimore, Md. : 1950) 2017 May 15;198(10):3989-3998
Journal of immunology (Baltimore, Md. : 1950) 2017 May 15;198(10):3989-3998
Key Residues at Third CDR3β Position Impact Structure and Antigen Recognition of Human Invariant NK TCRs.
Chamoto K, Guo T, Scally SW, Kagoya Y, Anczurowski M, Wang CH, Rahman MA, Saso K, Butler MO, Chiu PP, Julien JP, Hirano N
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 1;198(3):1056-1065
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 1;198(3):1056-1065
Functional characterization of human T cell hyporesponsiveness induced by CTLA4-Ig.
Rochman Y, Yukawa M, Kartashov AV, Barski A
PloS one 2015;10(4):e0122198
PloS one 2015;10(4):e0122198
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Pfleger C, Schloot N, ter Veld F
Journal of immunological methods 2008 Jan 1;329(1-2):214-8
Journal of immunological methods 2008 Jan 1;329(1-2):214-8
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS (London, England) 2007 Jun 19;21(10):1263-72
AIDS (London, England) 2007 Jun 19;21(10):1263-72
Th2 cytokine response in Major Depressive Disorder patients before treatment.
Pavón L, Sandoval-López G, Eugenia Hernández M, Loría F, Estrada I, Pérez M, Moreno J, Avila U, Leff P, Antón B, Heinze G
Journal of neuroimmunology 2006 Mar;172(1-2):156-65
Journal of neuroimmunology 2006 Mar;172(1-2):156-65
Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL
Journal of immunology (Baltimore, Md. : 1950) 2000 May 15;164(10):5337-43
Journal of immunology (Baltimore, Md. : 1950) 2000 May 15;164(10):5337-43
Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4.
Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL
Journal of immunology (Baltimore, Md. : 1950) 2000 May 15;164(10):5337-43
Journal of immunology (Baltimore, Md. : 1950) 2000 May 15;164(10):5337-43
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-2 in Human PBMCs. IL-2 was detected in immersion fixed PMA-, ionomycin-, and monensin-stimulated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human/Primate IL-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF202) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-2 in Human PBMCs. IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 15 µg/mL Goat Anti-Human/Primate IL-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF202) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
