Antibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-294-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal HGF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human HGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse HGF is observed. In Western blots, no cross-reactivity with recombinant canine HGF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Blocking the proteolytic activity of zymogen matriptase with antibody-based inhibitors.
Coronary stents with inducible VEGF/HGF-secreting UCB-MSCs reduced restenosis and increased re-endothelialization in a swine model.
Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury.
Matriptase regulates c-Met mediated proliferation and invasion in inflammatory breast cancer.
Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
Spontaneous genomic alterations in a chimeric model of colorectal cancer enable metastasis and guide effective combinatorial therapy.
Functional mononucleotide repeat polymorphism in the promoter region of HGF is associated with risk and malignant aggressiveness of bladder cancer.
Combined targeting of mTOR and c-MET signaling pathways for effective management of epithelioid sarcoma.
Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
Associations between hepatocyte growth factor, c-Met, and basic fibroblast growth factor and survival in endometrial cancer patients.
Heparanase plays a dual role in driving hepatocyte growth factor (HGF) signaling by enhancing HGF expression and activity.
c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
Wnt activity defines colon cancer stem cells and is regulated by the microenvironment.
Alternative proteolytic processing of hepatocyte growth factor during wound repair.
IFATS collection: Fibroblast growth factor-2-induced hepatocyte growth factor secretion by adipose-derived stromal cells inhibits postinjury fibrogenesis through a c-Jun N-terminal kinase-dependent mechanism.
Endogenous hepatocyte growth factor is a niche signal for subventricular zone neural stem cell amplification and self-renewal.
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Hepatocyte growth factor acts as a motogen and guidance signal for gonadotropin hormone-releasing hormone-1 neuronal migration.
Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome.
Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1.
Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1.
Tamberg T, Hong Z, De Schepper D, Skovbjerg S, Dupont DM, Vitved L, Schar CR, Skjoedt K, Vogel LK, Jensen JK
The Journal of biological chemistry 2019 Jan 4;294(1):314-326
The Journal of biological chemistry 2019 Jan 4;294(1):314-326
Coronary stents with inducible VEGF/HGF-secreting UCB-MSCs reduced restenosis and increased re-endothelialization in a swine model.
Chang HK, Kim PH, Kim DW, Cho HM, Jeong MJ, Kim DH, Joung YK, Lim KS, Kim HB, Lim HC, Han DK, Hong YJ, Cho JY
Experimental & molecular medicine 2018 Sep 3;50(9):114
Experimental & molecular medicine 2018 Sep 3;50(9):114
Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury.
Nagashima K, Miwa T, Soumiya H, Ushiro D, Takeda-Kawaguchi T, Tamaoki N, Ishiguro S, Sato Y, Miyamoto K, Ohno T, Osawa M, Kunisada T, Shibata T, Tezuka KI, Furukawa S, Fukumitsu H
Scientific reports 2017 Oct 18;7(1):13500
Scientific reports 2017 Oct 18;7(1):13500
Matriptase regulates c-Met mediated proliferation and invasion in inflammatory breast cancer.
Zoratti GL, Tanabe LM, Hyland TE, Duhaime MJ, Colombo É, Leduc R, Marsault E, Johnson MD, Lin CY, Boerner J, Lang JE, List K
Oncotarget 2016 Sep 6;7(36):58162-58173
Oncotarget 2016 Sep 6;7(36):58162-58173
Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
Zoratti GL, Tanabe LM, Varela FA, Murray AS, Bergum C, Colombo É, Lang JE, Molinolo AA, Leduc R, Marsault E, Boerner J, List K
Nature communications 2015 Apr 15;6:6776
Nature communications 2015 Apr 15;6:6776
Spontaneous genomic alterations in a chimeric model of colorectal cancer enable metastasis and guide effective combinatorial therapy.
Zhou Y, Rideout WM 3rd, Bressel A, Yalavarthi S, Zi T, Potz D, Farlow S, Brodeur J, Monti A, Reddipalli S, Xiao Q, Bottega S, Feng B, Chiu MI, Bosenberg M, Heyer J
PloS one 2014;9(8):e105886
PloS one 2014;9(8):e105886
Functional mononucleotide repeat polymorphism in the promoter region of HGF is associated with risk and malignant aggressiveness of bladder cancer.
Chiba S, Tsuchiya N, Horikawa Y, Narita S, Inoue T, Akihama S, Saito M, Numakura K, Tsuruta H, Huang M, Satoh S, Habuchi T
International journal of oncology 2014 Mar;44(3):678-84
International journal of oncology 2014 Mar;44(3):678-84
Combined targeting of mTOR and c-MET signaling pathways for effective management of epithelioid sarcoma.
Imura Y, Yasui H, Outani H, Wakamatsu T, Hamada K, Nakai T, Yamada S, Myoui A, Araki N, Ueda T, Itoh K, Yoshikawa H, Naka N
Molecular cancer 2014 Aug 7;13:185
Molecular cancer 2014 Aug 7;13:185
Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, Müller I, Mack A, Schumann S, Bühring HJ, Lang F, Sorg RV, Langer H, Gawaz M
The Journal of biological chemistry 2014 Apr 18;289(16):11068-82
The Journal of biological chemistry 2014 Apr 18;289(16):11068-82
Associations between hepatocyte growth factor, c-Met, and basic fibroblast growth factor and survival in endometrial cancer patients.
Felix AS, Edwards RP, Stone RA, Chivukula M, Parwani AV, Bowser R, Linkov F, Weissfeld JL
British journal of cancer 2012 Jun 5;106(12):2004-9
British journal of cancer 2012 Jun 5;106(12):2004-9
Heparanase plays a dual role in driving hepatocyte growth factor (HGF) signaling by enhancing HGF expression and activity.
Ramani VC, Yang Y, Ren Y, Nan L, Sanderson RD
The Journal of biological chemistry 2011 Feb 25;286(8):6490-9
The Journal of biological chemistry 2011 Feb 25;286(8):6490-9
c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
Szabo R, Rasmussen AL, Moyer AB, Kosa P, Schafer JM, Molinolo AA, Gutkind JS, Bugge TH
Oncogene 2011 Apr 28;30(17):2003-16
Oncogene 2011 Apr 28;30(17):2003-16
Wnt activity defines colon cancer stem cells and is regulated by the microenvironment.
Vermeulen L, De Sousa E Melo F, van der Heijden M, Cameron K, de Jong JH, Borovski T, Tuynman JB, Todaro M, Merz C, Rodermond H, Sprick MR, Kemper K, Richel DJ, Stassi G, Medema JP
Nature cell biology 2010 May;12(5):468-76
Nature cell biology 2010 May;12(5):468-76
Alternative proteolytic processing of hepatocyte growth factor during wound repair.
Buchstein N, Hoffmann D, Smola H, Lang S, Paulsson M, Niemann C, Krieg T, Eming SA
The American journal of pathology 2009 Jun;174(6):2116-28
The American journal of pathology 2009 Jun;174(6):2116-28
IFATS collection: Fibroblast growth factor-2-induced hepatocyte growth factor secretion by adipose-derived stromal cells inhibits postinjury fibrogenesis through a c-Jun N-terminal kinase-dependent mechanism.
Suga H, Eto H, Shigeura T, Inoue K, Aoi N, Kato H, Nishimura S, Manabe I, Gonda K, Yoshimura K
Stem cells (Dayton, Ohio) 2009 Jan;27(1):238-49
Stem cells (Dayton, Ohio) 2009 Jan;27(1):238-49
Endogenous hepatocyte growth factor is a niche signal for subventricular zone neural stem cell amplification and self-renewal.
Nicoleau C, Benzakour O, Agasse F, Thiriet N, Petit J, Prestoz L, Roger M, Jaber M, Coronas V
Stem cells (Dayton, Ohio) 2009 Feb;27(2):408-19
Stem cells (Dayton, Ohio) 2009 Feb;27(2):408-19
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Skibinski G, Elborn JS, Ennis M
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L69-76
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L69-76
Hepatocyte growth factor acts as a motogen and guidance signal for gonadotropin hormone-releasing hormone-1 neuronal migration.
Giacobini P, Messina A, Wray S, Giampietro C, Crepaldi T, Carmeliet P, Fasolo A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Jan 10;27(2):431-45
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Jan 10;27(2):431-45
Hepatocyte growth factor in polymorphonuclear leukocytes is increased in patients with systemic inflammatory response syndrome.
Matsushima A, Ogura H, Koh T, Fujita K, Yoshiya K, Sumi Y, Hosotsubo H, Kuwagata Y, Tanaka H, Shimazu T, Sugimoto H
The Journal of trauma 2004 Feb;56(2):259-64
The Journal of trauma 2004 Feb;56(2):259-64
Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1.
Kawai A, Naito N, Yoshida A, Morimoto Y, Ouchida M, Shimizu K, Beppu Y
Cancer letters 2004 Feb 10;204(1):105-13
Cancer letters 2004 Feb 10;204(1):105-13
Establishment and characterization of a biphasic synovial sarcoma cell line, SYO-1.
Kawai A, Naito N, Yoshida A, Morimoto Y, Ouchida M, Shimizu K, Beppu Y
Cancer letters 2004 Feb 10;204(1):105-13
Cancer letters 2004 Feb 10;204(1):105-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Recombinant Human HGF by Western Blot. Western blot shows 25 ng of Recombinant Human HGF (Catalog # 294-HG), Recombinant Mouse HGF (Catalog # 2207-HG) and Recombinant Canine HGF (Catalog # 3386-HG). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for HGF at approximately 30 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- HGF in Human Liver. HGF was detected in formalin fixed paraffin-embedded sections of human liver using 15 µg/mL Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-11 Secretion Induced by HGF and Neutralization by Human HGF Antibody. Recombinant Human HGF induces IL-11 secretion in the Saos-2 human osteosarcoma cell line. The dose-dependent response (orange line) is measured by the Human IL-11 Duoset (Catalog # DY218). Under these conditions, IL-11 secretion elicited by HGF is neutralized (green line) by increasing concentrations of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibod (Catalog # AF-294-NA). The ND50 is typically 0.01-0.06 μg/mL.