AF2207

antibody from R&D Systems

Targeting: HGF DFNB39, F-TCF, HGFB, HPTA, SF

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2207

Provider

R&D Systems

Product name

Mouse HGF Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse HGF in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) HGF, rhHGF R, recombinant mouse (rm) HGF R, rhHGF-A, and rmHGF-A is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q53WS5


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Epigenetic regulation of HGF/Met receptor axis is critical for the outgrowth of bone metastasis from breast carcinoma.

Bendinelli P, Maroni P, Matteucci E, Desiderio MA
Cell death & disease 2017 Feb 2;8(2):e2578

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Progesterone dose-dependently modulates hepatocyte growth factor production in 3T3-L1 mouse preadipocytes.

Ito T, Yamaji D, Kamikawa A, Abd Eldaim MA, Okamatsu-Ogura Y, Terao A, Saito M, Kimura K
Endocrine journal 2017 Aug 30;64(8):777-785

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Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma.

Roy A, Femel J, Huijbers EJ, Spillmann D, Larsson E, Ringvall M, Olsson AK, Åbrink M
PloS one 2016;11(5):e0156151

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MET is required for the recruitment of anti-tumoural neutrophils.

Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson AA, Wauters E, Walmsley S, Prenen H, Granot Z, Casazza A, Mazzone M
Nature 2015 Jun 18;522(7556):349-53

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Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.

Vogel S, Börger V, Peters C, Förster M, Liebfried P, Metzger K, Meisel R, Däubener W, Trapp T, Fischer JC, Gawaz M, Sorg RV
Cell death and differentiation 2015 Jul;22(7):1219-30

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Bone marrow progenitor cell therapy-mediated paracrine regulation of cardiac miRNA-155 modulates fibrotic response in diabetic hearts.

Kishore R, Verma SK, Mackie AR, Vaughan EE, Abramova TV, Aiko I, Krishnamurthy P
PloS one 2013;8(4):e60161

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Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis.

Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H
Cancer research 2013 Apr 15;73(8):2659-70

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Enhanced c-Met activity promotes G-CSF-induced mobilization of hematopoietic progenitor cells via ROS signaling.

Tesio M, Golan K, Corso S, Giordano S, Schajnovitz A, Vagima Y, Shivtiel S, Kalinkovich A, Caione L, Gammaitoni L, Laurenti E, Buss EC, Shezen E, Itkin T, Kollet O, Petit I, Trumpp A, Christensen J, Aglietta M, Piacibello W, Lapidot T
Blood 2011 Jan 13;117(2):419-28

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MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.

Narasaraju T, Ng HH, Phoon MC, Chow VT
American journal of respiratory cell and molecular biology 2010 Jun;42(6):732-43

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c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74.

Gordin M, Tesio M, Cohen S, Gore Y, Lantner F, Leng L, Bucala R, Shachar I
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 15;185(4):2020-31

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Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.

Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
The American journal of pathology 2006 Nov;169(5):1767-83

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

HGF in Mouse Skeletal Muscle. HGF was detected in perfusion fixed frozen sections of mouse skeletal muscle using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

HGF in Mouse Liver. HGF was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.