DPAB-GS01

antibody from Creative Diagnostics

Targeting: HGF DFNB39, F-TCF, HGFB, HPTA, SF

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Antibody Data

Product number

DPAB-GS01

Provider

Creative Diagnostics

Product name

Anti-HGF polyclonal antibody [Biotin]

Antibody type

Monoclonal

Description

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide?linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%?94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF-dependent c-MET activation is implicated in the development of many human cancers. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription. This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis. HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions. In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium.

Reactivity

Human

Host

Goat

Conjugate

Biotin

Isotype

IgG

Vial size

100ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution.6 months, -20 to -70°C under sterile conditions after reconstitu