H00002056-M02

antibody from Abnova Corporation

Targeting: EPO EP

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002056-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002056-M02, RRID:AB_11188758

Product name

EPO monoclonal antibody (M02), clone 1B12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant EPO.

Antigen sequence

MGVHECPAWLWLLLSLLSLPLGLPVLGAPPRLICD
SRVLERYLLEAKEAENITTGCAEHCSLNENITVPD
TKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQ
ALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALG
AQKEAISPPDAASAAPLRTITADTFRKLFRVYSNF
LRGKLKLYTGEACRTGDR


Isotype

IgG

Antibody clone number

1B12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of EPO expression in transfected 293T cell line by EPO monoclonal antibody (M02), clone 1B12.Lane 1: EPO transfected lysate (Predicted MW: 21.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged EPO is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol