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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB9591-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Erythropoietin/EPO Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Erythropoietin/EPO in direct ELISAs. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant rat Erythropoietin/EPO, 5-15% cross-reactivity with recombinant human Erythropoietin/EPO, and no cross-reactivity with recombinant mouse Thrombopoietin is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by Erythropoietin and Neutralization by Mouse Erythropoietin Antibody. Recombinant Mouse Erythropoietin (Catalog # 959-ME) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recom-binant Mouse Erythropoietin (10 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse Erythropoietin Monoclonal Antibody (Catalog # MAB9591). The ND50 is typically 0.15-0.75 µg/mL.
