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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
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- Product number
- PA5-77965 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Epo Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Recombinant human EPO protein Predicted Reactivity: Mouse (80%), Dog (81%), Rabbit (81%), Rhesus Monkey (90%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Epo in recombinant human EPO protein (with glycosylation) expressed in CHO cells using 20-100 ng of protein. Samples were separated with 12% SDS-PAGE and incubated with Epo polyclonal antibody (Product # PA5-77965) using a dilution of 1:20,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Epo Polyclonal Antibody (Product # PA5-77965). Recombinant human EPO protein (with glycosylation) expressed in CHO cells (20 and 100 ng) were separated by 12% SDS-PAGE, and the membrane was blotted with Epo Polyclonal Antibody (Product # PA5-77965) diluted at 1:20,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- An ELISA plate is coated with 50 µL of EPO recombinant protein at concentration ranged from 0.125 µg/mL to 4 µg/mL. The coated protein is detected with Epo Polyclonal Antibody (Product # PA5-77965) at 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Epo Polyclonal Antibody detects EPO protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Epo Polyclonal Antibody (Product # PA5-77965) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Epo Polyclonal Antibody detects EPO protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Epo Polyclonal Antibody (Product # PA5-77965) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
