Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-85107 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Epo Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Rat (80%), Dog (80%), Cat (82%), Pig (81%), Rabbit (80%), Sheep (81%), Rhesus Monkey (89%), Bovine (80%). Positive Control: rPT EPO. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.62 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPO in CHO cells using EPO polyclonal antibody (Product # PA5-85107) using 100 ng of sample at a dilution of 1:10000. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Epo Polyclonal Antibody (Product # PA5-85107). Recombinant human EPO protein (with glycosylation) expressed in CHO cells (100 ng) was separated by 12% SDS-PAGE, and the membrane was blotted with Epo Polyclonal Antibody Epo Polyclonal Antibody (Product # PA5-85107) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- An ELISA plate is coated with 50 µL of EPO recombinant protein at concentration ranged from 0.125 µg/mL to 4 µg/mL. The coated protein is detected with Epo Polyclonal Antibody (Product # PA5-85107) at 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Epo Polyclonal Antibody detects EPO protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Epo Polyclonal Antibody (Product # PA5-85107) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.