sc-823

GNAS antibody from Santa Cruz Biotechnology

GNAS1, GNASXL, GPSA, NESP, NESP55, SCG6, SgVI

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for sc-823

Antibody Data

Product number

sc-823

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-823, RRID:AB_631538

Product name

Anti-GNAS

Provider product page

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

A novel alternatively spliced isoform of the mu-opioid receptor: functional antagonism.
Gris P, Gauthier J, Cheng P, Gibson DG, Gris D, Laur O, Pierson J, Wentworth S, Nackley AG, Maixner W, Diatchenko L
Molecular pain 2010 Jun 2;6:33

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Coordinate down-regulation of adenylyl cyclase isoforms and the stimulatory G protein (G(s)) in intestinal epithelial cell differentiation.
Choi LJ, Jenikova G, Hanson E, Spehlmann ME, Boehling NS, Kirstein SL, Bundey RA, Smith JR, Insel PA, Eckmann L
The Journal of biological chemistry 2010 Apr 23;285(17):12504-11

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Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct.
Yu MJ, Pisitkun T, Wang G, Aranda JF, Gonzales PA, Tchapyjnikov D, Shen RF, Alonso MA, Knepper MA
American journal of physiology. Cell physiology 2008 Sep;295(3):C661-78

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Activation of the progesterone-signaling pathway by methyl-beta-cyclodextrin or steroid in Xenopus laevis oocytes involves release of 45-kDa Galphas.
Sadler SE, Archer MR, Spellman KM
Developmental biology 2008 Oct 1;322(1):199-207

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Alpha1-adrenergic receptors prevent a maladaptive cardiac response to pressure overload.
O'Connell TD, Swigart PM, Rodrigo MC, Ishizaka S, Joho S, Turnbull L, Tecott LH, Baker AJ, Foster E, Grossman W, Simpson PC
The Journal of clinical investigation 2006 Apr;116(4):1005-15

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 47

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.