TA809325

antibody from OriGene

Targeting: GNAS GNAS1, GNASXL, GPSA, NESP, NESP55, SCG6, SgVI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA809325

Provider

OriGene

Product name

GNAS mouse monoclonal antibody,clone OTI13G5

Antibody type

Monoclonal

Description

GNAS mouse monoclonal antibody,clone OTI13G5

Host

Mouse

Conjugate

Unconjugated

Epitope

GNAS

Isotype

IgG

Antibody clone number

OTI13G5

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GNAS (RC214197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNAS.(1:2000)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-GNAS monoclonal antibody . (1:500)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GNAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809325)(1:2000)

Validation comment

IHC