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LearnPA5-42229
antibody from Invitrogen Antibodies
Targeting: GNAS
GNAS1, GNASXL, GPSA, NESP, NESP55, SCG6, SgVI
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
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- Product number
- PA5-42229 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GNAS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: VYRATHRLLL LGAGESGKST IVKQMRILHV NGFNGEGGEE DPQAARSNSD
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of INS1 cell lysate using an anti-GNAS polyclonal antibody (Product # PA5-42229). Primary Antibody Dilution: 1:1000; Secondary Antibody: Donkey anti-rabbit-HRP; Secondary Antibody Dilution: 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MCF7 whole cell lysate using an anti-GNAS polyclonal antibody (Product # PA5-42229). Lane 1) Primary Antibody; Lane 2) Primary Antibody + Blocking Peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MCF7 cell lysate using an anti-GNAS polyclonal antibody (Product # PA5-42229).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GNAS Polyclonal Antibody (Product # PA5-42229) and a 46kDa band corresponding to GNAS was observed in Mouse Brain and Rat Brain, but not in Mouse Adipose which is reported to be negative. Whole cell lysate (30ug lysate) of Mouse Brain (Lane 1), Mouse Adipose (Lane 2) and Rat Brain (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human thyroid tissue using an anti-GNAS polyclonal antibody (Product # PA5-42229).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human thyroid tissue using an anti-GNAS polyclonal antibody (Product # PA5-42229).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human pancreas tissue using an anti-GNAS polyclonal antibody (Product # PA5-42229).
