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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [102]
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- Validations
- Western blot [1]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- M701 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFN gamma Monoclonal Antibody (B133.5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M701 was successfully to detect IFN-gamma in Flow application. M701 targets IFN gamma in Western blot applications and shows reactivity with Human samples. The M700A anti-IFN Gamma antibody (clone 2G1) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M701B (biotinylated conjugate of clone B133.5). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. The M701 immunogen is recombinant human IFN gamma. M701 detects IFN gamma which has a predicted molecular weight of approximately 16 kDa. This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- B133.5
- Vial size
- 500 µg
- Concentration
- 1.0 mg/mL
- Storage
- -20°C
Submitted references Preformed donor-reactive T cells that persist after ABO desensitization predict severe T cell-mediated rejection after living donor kidney transplantation - a retrospective study.
Molecular affinity rulers: systematic evaluation of DNA aptamers for their applicabilities in ELISA.
Transplantectomy is associated with presensitization with donor-reactive T cells and graft failure after kidney retransplantation: a cohort study.
Bee venom processes human skin lipids for presentation by CD1a.
Inflammatory activation and recovering BKV-specific immunity correlate with self-limited BKV replication after renal transplantation.
Modular multiantigen T cell epitope-enriched DNA vaccine against human leishmaniasis.
Innate immune control of EBV-infected B cells by invariant natural killer T cells.
Imaging of Protein Secretion from a Single Cell Using Plasmonic Substrates.
Long-term proliferation of functional human NK cells, with conversion of CD56(dim) NK cells to a CD56 (bright) phenotype, induced by carcinoma cells co-expressing 4-1BBL and IL-12.
Chemically programmed bispecific antibodies that recruit and activate T cells.
Integrated immunoassay using tuneable surface acoustic waves and lensfree detection.
Antibody and Th1-type cell-mediated immune responses in elderly and young adults immunized with the standard or a high dose influenza vaccine.
Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.
Increased per cell IFN-gamma productivity indicates recent in vivo activation of T cells.
Mycophenolic acid inhibits maturation and function of human dendritic cells and B cells.
Apolipoprotein-mediated lipid antigen presentation in B cells provides a pathway for innate help by NKT cells.
Recombinant bacillus Calmette-Guérin (BCG) expressing interferon-alpha 2B enhances human mononuclear cell cytotoxicity against bladder cancer cell lines in vitro.
Cell-mediated immunity elicited by the blood stage malaria vaccine apical membrane antigen 1 in Malian adults: results of a Phase I randomized trial.
Natalizumab treatment is associated with peripheral sequestration of proinflammatory T cells.
Expression of immune modulator cytokines in human fulminant amoebic colitis.
Multiplexed Elispot assay.
Lack of disease specificity limits the usefulness of in vitro costimulation in HIV- and HCV-infected patients.
The TRAIL of helpless CD8+ T cells in HIV infection.
Measurement of varicella-zoster virus (VZV)-specific cell-mediated immunity: comparison between VZV skin test and interferon-gamma enzyme-linked immunospot assay.
Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases.
Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.
HLA class I-restricted T cell epitopes of the kinetoplastid membrane protein-11 presented by Leishmania donovani-infected human macrophages.
Dose-dependent synergy of Th1-stimulating cytokines on bacille Calmette-Guérin-induced interferon-gamma production by human mononuclear cells.
JKT-1 is not a human seminoma cell line.
Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination.
Immunomodulation in type 1 diabetes by NBI-6024, an altered peptide ligand of the insulin B epitope.
IgE-activated mast cells in combination with pro-inflammatory factors induce Th2-promoting dendritic cells.
HLA-A2 supertype-restricted cell-mediated immunity by peripheral blood mononuclear cells derived from Malian children with severe or uncomplicated Plasmodium falciparum malaria and healthy controls.
Hepatitis C virus-specific reactivity of CD4+-lymphocytes in children born from HCV-infected women.
IL-2 absorption affects IFN-gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays.
Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.
Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.
Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation.
Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation.
Phenotypic and functional analysis of EBV-specific memory CD8 cells in SLE.
Immunization of volunteers with Salmonella enterica serovar Typhi strain Ty21a elicits the oligoclonal expansion of CD8+ T cells with predominant Vbeta repertoires.
Interferon (IFN)-gamma is a main mediator of keratinocyte (HaCaT) apoptosis and contributes to autocrine IFN-gamma and tumour necrosis factor-alpha production.
Donor-specific hyporesponsiveness in ELISPOT assay is associated with early recurrence of hepatitis C in liver transplant recipients.
Human mesenchymal stem cells require monocyte-mediated activation to suppress alloreactive T cells.
Active specific immunotherapy of melanoma with a GM3 ganglioside-based vaccine: a report on safety and immunogenicity.
Active specific immunotherapy of melanoma with a GM3 ganglioside-based vaccine: a report on safety and immunogenicity.
Human mesenchymal stem cells support unrelated donor hematopoietic stem cells and suppress T-cell activation.
Cytokine and chemokine receptor profile of peripheral blood mononuclear cells during treatment with infliximab in patients with active rheumatoid arthritis.
Impeded Th1 CD4 memory T cell generation in chronic-persisting liver infection with Echinococcus multilocularis.
Impeded Th1 CD4 memory T cell generation in chronic-persisting liver infection with Echinococcus multilocularis.
Proteasomal targeting and minigene repetition improve cell-surface presentation of a transfected, modified melanoma tumour antigen.
Proteasomal targeting and minigene repetition improve cell-surface presentation of a transfected, modified melanoma tumour antigen.
Tc1 effector diversity shows dissociated expression of granzyme B and interferon-gamma in HIV infection.
Tc1 effector diversity shows dissociated expression of granzyme B and interferon-gamma in HIV infection.
CD28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen-specific memory effector CD4 and CD8 cell populations in human diseases.
Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.
Human T-cells recognise N-terminally Fmoc-modified peptide.
Human T-cells recognise N-terminally Fmoc-modified peptide.
Concomitant induction of CD4+ and CD8+ T cell responses in volunteers immunized with Salmonella enterica serovar typhi strain CVD 908-htrA.
Concomitant induction of CD4+ and CD8+ T cell responses in volunteers immunized with Salmonella enterica serovar typhi strain CVD 908-htrA.
Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.
Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.
CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.
CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.
Squamous cell carcinoma cells differentially stimulate NK cell effector functions: the role of IL-18.
Serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women.
Monitoring indirect presentation of alloantigens by utilizing the autologous processing machinery of dendritic cells in-vitro.
Monitoring indirect presentation of alloantigens by utilizing the autologous processing machinery of dendritic cells in-vitro.
Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.
Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.
Interferon--gamma control of EBV-transformed B cells: a role for CD8+ T cells that poorly kill EBV-infected cells.
Age-dependent cellular immune responses to Plasmodium vivax Duffy binding protein in humans.
Age-dependent cellular immune responses to Plasmodium vivax Duffy binding protein in humans.
Differing influences of virus burden and immune activation on disease severity in secondary dengue-3 virus infections.
A HLA-A2 restricted human CTL line recognizes a novel tumor cell expressed p53 epitope.
Characterization of CD8(+) effector T cell responses in volunteers immunized with Salmonella enterica serovar Typhi strain Ty21a typhoid vaccine.
Characterization of CD8(+) effector T cell responses in volunteers immunized with Salmonella enterica serovar Typhi strain Ty21a typhoid vaccine.
Inhibition of human immunodeficiency virus type 1 gp120 presentation to CD4 T cells by antibodies specific for the CD4 binding domain of gp120.
Schistosoma haematobium-induced urinary tract morbidity correlates with increased tumor necrosis factor-alpha and diminished interleukin-10 production.
Adaptive immune responses of patients with asthma to the attachment (G) glycoprotein of respiratory synctial virus.
A T cell clone's avidity is a function of its activation state.
A T cell clone's avidity is a function of its activation state.
IL-10 secretion and sensitivity in normal human intestine and inflammatory bowel disease.
IL-10 secretion and sensitivity in normal human intestine and inflammatory bowel disease.
Immune responses to hepatitis C and non-hepatitis C antigens in hepatitis C virus infected and HIV-1 coinfected patients.
Immune responses to hepatitis C and non-hepatitis C antigens in hepatitis C virus infected and HIV-1 coinfected patients.
CD40-ligand (CD154) gene therapy for chronic lymphocytic leukemia.
Naturally developing memory T cell xenoreactivity to swine antigens in human peripheral blood lymphocytes.
Helminth- and Bacillus Calmette-Guérin-induced immunity in children sensitized in utero to filariasis and schistosomiasis.
Pretransplant frequency of donor-specific, IFN-gamma-producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes.
T-cell anergy induced by clonotype-specific antibodies: modulation of an autoreactive human T-cell clone in vitro.
T-cell anergy induced by clonotype-specific antibodies: modulation of an autoreactive human T-cell clone in vitro.
Donor-specific stimulation of peripheral blood mononuclear cells from recipients of orthotopic liver transplants is associated, in the absence of rejection, with type-2 cytokine production.
Down-regulation of CD1 on antigen-presenting cells by infection with Mycobacterium tuberculosis.
Increased interleukin-4 and interleukin-5 production in response to Schistosoma haematobium adult worm antigens correlates with lack of reinfection after treatment.
Increased interleukin-4 and interleukin-5 production in response to Schistosoma haematobium adult worm antigens correlates with lack of reinfection after treatment.
Ligation of TAPA-1 (CD81) or major histocompatibility complex class II in co-cultures of human B and T lymphocytes enhances interleukin-4 synthesis by antigen-specific CD4+ T cells.
IL-12 synthesis by human Langerhans cells.
Interleukin 12 synergizes with B7/CD28 interaction in inducing efficient proliferation and cytokine production of human T cells.
Interleukin 12 synergizes with B7/CD28 interaction in inducing efficient proliferation and cytokine production of human T cells.
Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells.
Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells.
Schachtner T, Stein M, Otto NM, Reinke P
Transplant international : official journal of the European Society for Organ Transplantation 2020 Mar;33(3):288-297
Transplant international : official journal of the European Society for Organ Transplantation 2020 Mar;33(3):288-297
Molecular affinity rulers: systematic evaluation of DNA aptamers for their applicabilities in ELISA.
Kimoto M, Shermane Lim YW, Hirao I
Nucleic acids research 2019 Sep 19;47(16):8362-8374
Nucleic acids research 2019 Sep 19;47(16):8362-8374
Transplantectomy is associated with presensitization with donor-reactive T cells and graft failure after kidney retransplantation: a cohort study.
Schachtner T, Otto NM, Stein M, Reinke P
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2018 May 1;33(5):889-896
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2018 May 1;33(5):889-896
Bee venom processes human skin lipids for presentation by CD1a.
Bourgeois EA, Subramaniam S, Cheng TY, De Jong A, Layre E, Ly D, Salimi M, Legaspi A, Modlin RL, Salio M, Cerundolo V, Moody DB, Ogg G
The Journal of experimental medicine 2015 Feb 9;212(2):149-63
The Journal of experimental medicine 2015 Feb 9;212(2):149-63
Inflammatory activation and recovering BKV-specific immunity correlate with self-limited BKV replication after renal transplantation.
Schachtner T, Stein M, Sefrin A, Babel N, Reinke P
Transplant international : official journal of the European Society for Organ Transplantation 2014 Mar;27(3):290-301
Transplant international : official journal of the European Society for Organ Transplantation 2014 Mar;27(3):290-301
Modular multiantigen T cell epitope-enriched DNA vaccine against human leishmaniasis.
Das S, Freier A, Boussoffara T, Das S, Oswald D, Losch FO, Selka M, Sacerdoti-Sierra N, Schönian G, Wiesmüller KH, Seifert K, Schroff M, Juhls C, Jaffe CL, Roy S, Das P, Louzir H, Croft SL, Modabber F, Walden P
Science translational medicine 2014 Apr 30;6(234):234ra56
Science translational medicine 2014 Apr 30;6(234):234ra56
Innate immune control of EBV-infected B cells by invariant natural killer T cells.
Chung BK, Tsai K, Allan LL, Zheng DJ, Nie JC, Biggs CM, Hasan MR, Kozak FK, van den Elzen P, Priatel JJ, Tan R
Blood 2013 Oct 10;122(15):2600-8
Blood 2013 Oct 10;122(15):2600-8
Imaging of Protein Secretion from a Single Cell Using Plasmonic Substrates.
Szmacinski H, Toshchakov V, Piao W, Lakowicz JR
BioNanoScience 2013 Mar 1;3(1):30-36
BioNanoScience 2013 Mar 1;3(1):30-36
Long-term proliferation of functional human NK cells, with conversion of CD56(dim) NK cells to a CD56 (bright) phenotype, induced by carcinoma cells co-expressing 4-1BBL and IL-12.
Dowell AC, Oldham KA, Bhatt RI, Lee SP, Searle PF
Cancer immunology, immunotherapy : CII 2012 May;61(5):615-28
Cancer immunology, immunotherapy : CII 2012 May;61(5):615-28
Chemically programmed bispecific antibodies that recruit and activate T cells.
Cui H, Thomas JD, Burke TR Jr, Rader C
The Journal of biological chemistry 2012 Aug 17;287(34):28206-14
The Journal of biological chemistry 2012 Aug 17;287(34):28206-14
Integrated immunoassay using tuneable surface acoustic waves and lensfree detection.
Bourquin Y, Reboud J, Wilson R, Zhang Y, Cooper JM
Lab on a chip 2011 Aug 21;11(16):2725-30
Lab on a chip 2011 Aug 21;11(16):2725-30
Antibody and Th1-type cell-mediated immune responses in elderly and young adults immunized with the standard or a high dose influenza vaccine.
Chen WH, Cross AS, Edelman R, Sztein MB, Blackwelder WC, Pasetti MF
Vaccine 2011 Apr 5;29(16):2865-73
Vaccine 2011 Apr 5;29(16):2865-73
Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.
Loewenbrueck KF, Tigno-Aranjuez JT, Boehm BO, Lehmann PV, Tary-Lehmann M
Neurobiology of aging 2010 Oct;31(10):1732-42
Neurobiology of aging 2010 Oct;31(10):1732-42
Increased per cell IFN-gamma productivity indicates recent in vivo activation of T cells.
Schlingmann TR, Shive CL, Targoni OS, Tary-Lehmann M, Lehmann PV
Cellular immunology 2009;258(2):131-7
Cellular immunology 2009;258(2):131-7
Mycophenolic acid inhibits maturation and function of human dendritic cells and B cells.
Wadia PP, Herrera ND, Abecassis MM, Tambur AR
Human immunology 2009 Sep;70(9):692-700
Human immunology 2009 Sep;70(9):692-700
Apolipoprotein-mediated lipid antigen presentation in B cells provides a pathway for innate help by NKT cells.
Allan LL, Hoefl K, Zheng DJ, Chung BK, Kozak FK, Tan R, van den Elzen P
Blood 2009 Sep 17;114(12):2411-6
Blood 2009 Sep 17;114(12):2411-6
Recombinant bacillus Calmette-Guérin (BCG) expressing interferon-alpha 2B enhances human mononuclear cell cytotoxicity against bladder cancer cell lines in vitro.
Liu W, O'Donnell MA, Chen X, Han R, Luo Y
Cancer immunology, immunotherapy : CII 2009 Oct;58(10):1647-55
Cancer immunology, immunotherapy : CII 2009 Oct;58(10):1647-55
Cell-mediated immunity elicited by the blood stage malaria vaccine apical membrane antigen 1 in Malian adults: results of a Phase I randomized trial.
Lyke KE, Daou M, Diarra I, Kone A, Kouriba B, Thera MA, Dutta S, Lanar DE, Heppner DG Jr, Doumbo OK, Plowe CV, Sztein MB
Vaccine 2009 Mar 26;27(15):2171-6
Vaccine 2009 Mar 26;27(15):2171-6
Natalizumab treatment is associated with peripheral sequestration of proinflammatory T cells.
Kivisäkk P, Healy BC, Viglietta V, Quintana FJ, Hootstein MA, Weiner HL, Khoury SJ
Neurology 2009 Jun 2;72(22):1922-30
Neurology 2009 Jun 2;72(22):1922-30
Expression of immune modulator cytokines in human fulminant amoebic colitis.
Sierra-Puente RE, Campos-Rodríguez R, Jarillo-Luna RA, Muñoz-Fernández L, Rodríguez MG, Muñoz-Ortega MH, Ventura-Juárez J
Parasite immunology 2009 Jul;31(7):384-91
Parasite immunology 2009 Jul;31(7):384-91
Multiplexed Elispot assay.
Harriman WD, Collarini EJ, Cromer RG, Dutta A, Strandh M, Zhang F, Kauvar LM
Journal of immunological methods 2009 Feb 28;341(1-2):127-34
Journal of immunological methods 2009 Feb 28;341(1-2):127-34
Lack of disease specificity limits the usefulness of in vitro costimulation in HIV- and HCV-infected patients.
Kuerten S, Schlingmann TR, Rajasalu T, Angelov DN, Lehmann PV, Tary-Lehmann M
Clinical & developmental immunology 2008;2008:590941
Clinical & developmental immunology 2008;2008:590941
The TRAIL of helpless CD8+ T cells in HIV infection.
Kuerten S, Asaad RJ, Schoenberger SP, Angelov DN, Lehmann PV, Tary-Lehmann M
AIDS research and human retroviruses 2008 Sep;24(9):1175-83
AIDS research and human retroviruses 2008 Sep;24(9):1175-83
Measurement of varicella-zoster virus (VZV)-specific cell-mediated immunity: comparison between VZV skin test and interferon-gamma enzyme-linked immunospot assay.
Sadaoka K, Okamoto S, Gomi Y, Tanimoto T, Ishikawa T, Yoshikawa T, Asano Y, Yamanishi K, Mori Y
The Journal of infectious diseases 2008 Nov 1;198(9):1327-33
The Journal of infectious diseases 2008 Nov 1;198(9):1327-33
Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases.
Marotti B, Rocco A, De Colibus P, Compare D, de Nucci G, Staibano S, Tatangelo F, Romano M, Nardone G
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 2008 Apr;40(4):240-7
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 2008 Apr;40(4):240-7
Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.
Nowacki TM, Kuerten S, Zhang W, Shive CL, Kreher CR, Boehm BO, Lehmann PV, Tary-Lehmann M
Cellular immunology 2007 May;247(1):36-48
Cellular immunology 2007 May;247(1):36-48
HLA class I-restricted T cell epitopes of the kinetoplastid membrane protein-11 presented by Leishmania donovani-infected human macrophages.
Basu R, Roy S, Walden P
The Journal of infectious diseases 2007 May 1;195(9):1373-80
The Journal of infectious diseases 2007 May 1;195(9):1373-80
Dose-dependent synergy of Th1-stimulating cytokines on bacille Calmette-Guérin-induced interferon-gamma production by human mononuclear cells.
Chen X, O'DONNELL MA, Luo Y
Clinical and experimental immunology 2007 Jul;149(1):178-85
Clinical and experimental immunology 2007 Jul;149(1):178-85
JKT-1 is not a human seminoma cell line.
de Jong J, Stoop H, Gillis AJ, van Gurp RJ, van Drunen E, Beverloo HB, Lau YF, Schneider DT, Sherlock JK, Baeten J, Hatakeyama S, Ohyama C, Oosterhuis JW, Looijenga LH
International journal of andrology 2007 Aug;30(4):350-65
International journal of andrology 2007 Aug;30(4):350-65
Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination.
Nagarajan S, Selvaraj P
Vaccine 2006 Mar 20;24(13):2264-74
Vaccine 2006 Mar 20;24(13):2264-74
Immunomodulation in type 1 diabetes by NBI-6024, an altered peptide ligand of the insulin B epitope.
Alleva DG, Maki RA, Putnam AL, Robinson JM, Kipnes MS, Dandona P, Marks JB, Simmons DL, Greenbaum CJ, Jimenez RG, Conlon PJ, Gottlieb PA
Scandinavian journal of immunology 2006 Jan;63(1):59-69
Scandinavian journal of immunology 2006 Jan;63(1):59-69
IgE-activated mast cells in combination with pro-inflammatory factors induce Th2-promoting dendritic cells.
Kitawaki T, Kadowaki N, Sugimoto N, Kambe N, Hori T, Miyachi Y, Nakahata T, Uchiyama T
International immunology 2006 Dec;18(12):1789-99
International immunology 2006 Dec;18(12):1789-99
HLA-A2 supertype-restricted cell-mediated immunity by peripheral blood mononuclear cells derived from Malian children with severe or uncomplicated Plasmodium falciparum malaria and healthy controls.
Lyke KE, Burges RB, Cissoko Y, Sangare L, Kone A, Dao M, Diarra I, Fernández-Vina MA, Plowe CV, Doumbo OK, Sztein MB
Infection and immunity 2005 Sep;73(9):5799-808
Infection and immunity 2005 Sep;73(9):5799-808
Hepatitis C virus-specific reactivity of CD4+-lymphocytes in children born from HCV-infected women.
Della Bella S, Riva A, Tanzi E, Nicola S, Amendola A, Vecchi L, Nebbia G, Longhi R, Zanetti AR, Villa ML
Journal of hepatology 2005 Sep;43(3):394-402
Journal of hepatology 2005 Sep;43(3):394-402
IL-2 absorption affects IFN-gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays.
Quast S, Zhang W, Shive C, Kovalovski D, Ott PA, Herzog BA, Boehm BO, Tary-Lehmann M, Karulin AY, Lehmann PV
Cellular immunology 2005 Sep;237(1):28-36
Cellular immunology 2005 Sep;237(1):28-36
Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.
Kuekrek H, Schlingmann T, Valdez H, Boehm BO, Pollard RB, Mitsuyasu R, Goebel FD, Lederman MM, Lehmann PV, Tary-Lehmann M
AIDS (London, England) 2005 Nov 18;19(17):1967-74
AIDS (London, England) 2005 Nov 18;19(17):1967-74
Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.
Kuekrek H, Schlingmann T, Valdez H, Boehm BO, Pollard RB, Mitsuyasu R, Goebel FD, Lederman MM, Lehmann PV, Tary-Lehmann M
AIDS (London, England) 2005 Nov 18;19(17):1967-74
AIDS (London, England) 2005 Nov 18;19(17):1967-74
Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation.
Kleen TO, Kadereit S, Fanning LR, Jaroscak J, Fu P, Meyerson HJ, Kulchycki L, Slivka LF, Kozik M, Tary-Lehmann M, Laughlin MJ
Transplantation 2005 Nov 15;80(9):1316-22
Transplantation 2005 Nov 15;80(9):1316-22
Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation.
Kleen TO, Kadereit S, Fanning LR, Jaroscak J, Fu P, Meyerson HJ, Kulchycki L, Slivka LF, Kozik M, Tary-Lehmann M, Laughlin MJ
Transplantation 2005 Nov 15;80(9):1316-22
Transplantation 2005 Nov 15;80(9):1316-22
Phenotypic and functional analysis of EBV-specific memory CD8 cells in SLE.
Berner BR, Tary-Lehmann M, Yonkers NL, Askari AD, Lehmann PV, Anthony DD
Cellular immunology 2005 May;235(1):29-38
Cellular immunology 2005 May;235(1):29-38
Immunization of volunteers with Salmonella enterica serovar Typhi strain Ty21a elicits the oligoclonal expansion of CD8+ T cells with predominant Vbeta repertoires.
Salerno-Gonçalves R, Wahid R, Sztein MB
Infection and immunity 2005 Jun;73(6):3521-30
Infection and immunity 2005 Jun;73(6):3521-30
Interferon (IFN)-gamma is a main mediator of keratinocyte (HaCaT) apoptosis and contributes to autocrine IFN-gamma and tumour necrosis factor-alpha production.
Konur A, Schulz U, Eissner G, Andreesen R, Holler E
The British journal of dermatology 2005 Jun;152(6):1134-42
The British journal of dermatology 2005 Jun;152(6):1134-42
Donor-specific hyporesponsiveness in ELISPOT assay is associated with early recurrence of hepatitis C in liver transplant recipients.
Tambur AR, Ben-Ari Z, Herrera ND, Klein T, Michowiz R, Mor E
Human immunology 2005 Jan;66(1):21-7
Human immunology 2005 Jan;66(1):21-7
Human mesenchymal stem cells require monocyte-mediated activation to suppress alloreactive T cells.
Groh ME, Maitra B, Szekely E, Koç ON
Experimental hematology 2005 Aug;33(8):928-34
Experimental hematology 2005 Aug;33(8):928-34
Active specific immunotherapy of melanoma with a GM3 ganglioside-based vaccine: a report on safety and immunogenicity.
Guthmann MD, Bitton RJ, Carnero AJ, Gabri MR, Cinat G, Koliren L, Lewi D, Fernandez LE, Alonso DF, Gómez DE, Fainboim L
Journal of immunotherapy (Hagerstown, Md. : 1997) 2004 Nov-Dec;27(6):442-51
Journal of immunotherapy (Hagerstown, Md. : 1997) 2004 Nov-Dec;27(6):442-51
Active specific immunotherapy of melanoma with a GM3 ganglioside-based vaccine: a report on safety and immunogenicity.
Guthmann MD, Bitton RJ, Carnero AJ, Gabri MR, Cinat G, Koliren L, Lewi D, Fernandez LE, Alonso DF, Gómez DE, Fainboim L
Journal of immunotherapy (Hagerstown, Md. : 1997) 2004 Nov-Dec;27(6):442-51
Journal of immunotherapy (Hagerstown, Md. : 1997) 2004 Nov-Dec;27(6):442-51
Human mesenchymal stem cells support unrelated donor hematopoietic stem cells and suppress T-cell activation.
Maitra B, Szekely E, Gjini K, Laughlin MJ, Dennis J, Haynesworth SE, Koç ON
Bone marrow transplantation 2004 Mar;33(6):597-604
Bone marrow transplantation 2004 Mar;33(6):597-604
Cytokine and chemokine receptor profile of peripheral blood mononuclear cells during treatment with infliximab in patients with active rheumatoid arthritis.
Nissinen R, Leirisalo-Repo M, Peltomaa R, Palosuo T, Vaarala O
Annals of the rheumatic diseases 2004 Jun;63(6):681-7
Annals of the rheumatic diseases 2004 Jun;63(6):681-7
Impeded Th1 CD4 memory T cell generation in chronic-persisting liver infection with Echinococcus multilocularis.
Manfras BJ, Reuter S, Wendland T, Boehm BO, Kern P
International immunology 2004 Jan;16(1):43-50
International immunology 2004 Jan;16(1):43-50
Impeded Th1 CD4 memory T cell generation in chronic-persisting liver infection with Echinococcus multilocularis.
Manfras BJ, Reuter S, Wendland T, Boehm BO, Kern P
International immunology 2004 Jan;16(1):43-50
International immunology 2004 Jan;16(1):43-50
Proteasomal targeting and minigene repetition improve cell-surface presentation of a transfected, modified melanoma tumour antigen.
Rasmussen AB, Zocca MB, Bonefeld CM, von Essen M, Lauritsen JP, Tomra S, Ødum N, Geisler C
Scandinavian journal of immunology 2004 Feb;59(2):220-7
Scandinavian journal of immunology 2004 Feb;59(2):220-7
Proteasomal targeting and minigene repetition improve cell-surface presentation of a transfected, modified melanoma tumour antigen.
Rasmussen AB, Zocca MB, Bonefeld CM, von Essen M, Lauritsen JP, Tomra S, Ødum N, Geisler C
Scandinavian journal of immunology 2004 Feb;59(2):220-7
Scandinavian journal of immunology 2004 Feb;59(2):220-7
Tc1 effector diversity shows dissociated expression of granzyme B and interferon-gamma in HIV infection.
Kleen TO, Asaad R, Landry SJ, Boehm BO, Tary-Lehmann M
AIDS (London, England) 2004 Feb 20;18(3):383-92
AIDS (London, England) 2004 Feb 20;18(3):383-92
Tc1 effector diversity shows dissociated expression of granzyme B and interferon-gamma in HIV infection.
Kleen TO, Asaad R, Landry SJ, Boehm BO, Tary-Lehmann M
AIDS (London, England) 2004 Feb 20;18(3):383-92
AIDS (London, England) 2004 Feb 20;18(3):383-92
CD28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen-specific memory effector CD4 and CD8 cell populations in human diseases.
Ott PA, Berner BR, Herzog BA, Guerkov R, Yonkers NL, Durinovic-Bello I, Tary-Lehmann M, Lehmann PV, Anthony DD
Journal of immunological methods 2004 Feb 15;285(2):223-35
Journal of immunological methods 2004 Feb 15;285(2):223-35
Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.
Schotte H, Schlüter B, Willeke P, Mickholz E, Schorat MA, Domschke W, Gaubitz M
Rheumatology (Oxford, England) 2004 Aug;43(8):960-4
Rheumatology (Oxford, England) 2004 Aug;43(8):960-4
Human T-cells recognise N-terminally Fmoc-modified peptide.
Mannering SI, Purcell AW, Honeyman MC, McCluskey J, Harrison LC
Vaccine 2003 Sep 8;21(25-26):3638-46
Vaccine 2003 Sep 8;21(25-26):3638-46
Human T-cells recognise N-terminally Fmoc-modified peptide.
Mannering SI, Purcell AW, Honeyman MC, McCluskey J, Harrison LC
Vaccine 2003 Sep 8;21(25-26):3638-46
Vaccine 2003 Sep 8;21(25-26):3638-46
Concomitant induction of CD4+ and CD8+ T cell responses in volunteers immunized with Salmonella enterica serovar typhi strain CVD 908-htrA.
Salerno-Gonçalves R, Wyant TL, Pasetti MF, Fernandez-Viña M, Tacket CO, Levine MM, Sztein MB
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2734-41
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2734-41
Concomitant induction of CD4+ and CD8+ T cell responses in volunteers immunized with Salmonella enterica serovar typhi strain CVD 908-htrA.
Salerno-Gonçalves R, Wyant TL, Pasetti MF, Fernandez-Viña M, Tacket CO, Levine MM, Sztein MB
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2734-41
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2734-41
Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.
Meloni F, Cascina A, Paschetto E, Marone Bianco A, Morosini M, Pellegrini C, Fietta A, Vitulo P, Pozzi E, Viganò M
Transplantation proceedings 2003 Jun;35(4):1523-6
Transplantation proceedings 2003 Jun;35(4):1523-6
Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.
Meloni F, Cascina A, Paschetto E, Marone Bianco A, Morosini M, Pellegrini C, Fietta A, Vitulo P, Pozzi E, Viganò M
Transplantation proceedings 2003 Jun;35(4):1523-6
Transplantation proceedings 2003 Jun;35(4):1523-6
CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.
Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M
Journal of immunological methods 2003 Jul;278(1-2):79-93
Journal of immunological methods 2003 Jul;278(1-2):79-93
CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.
Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M
Journal of immunological methods 2003 Jul;278(1-2):79-93
Journal of immunological methods 2003 Jul;278(1-2):79-93
Squamous cell carcinoma cells differentially stimulate NK cell effector functions: the role of IL-18.
Moore MB, Kurago ZB, Fullenkamp CA, Lutz CT
Cancer immunology, immunotherapy : CII 2003 Feb;52(2):107-15
Cancer immunology, immunotherapy : CII 2003 Feb;52(2):107-15
Serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women.
Tsunoda M, Litonjua AA, Kuniak MP, Weiss ST, Satoh T, Guevarra L, Tollerud DJ
International archives of allergy and immunology 2003 Feb;130(2):158-64
International archives of allergy and immunology 2003 Feb;130(2):158-64
Monitoring indirect presentation of alloantigens by utilizing the autologous processing machinery of dendritic cells in-vitro.
Tambur AR
Journal of immunological methods 2003 Dec;283(1-2):215-23
Journal of immunological methods 2003 Dec;283(1-2):215-23
Monitoring indirect presentation of alloantigens by utilizing the autologous processing machinery of dendritic cells in-vitro.
Tambur AR
Journal of immunological methods 2003 Dec;283(1-2):215-23
Journal of immunological methods 2003 Dec;283(1-2):215-23
Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.
Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, Lehmann PV, Schwander SK
Journal of immunological methods 2003 Aug;279(1-2):111-21
Journal of immunological methods 2003 Aug;279(1-2):111-21
Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.
Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, Lehmann PV, Schwander SK
Journal of immunological methods 2003 Aug;279(1-2):111-21
Journal of immunological methods 2003 Aug;279(1-2):111-21
Interferon--gamma control of EBV-transformed B cells: a role for CD8+ T cells that poorly kill EBV-infected cells.
Shi Y, Lutz CT
Viral immunology 2002;15(1):213-25
Viral immunology 2002;15(1):213-25
Age-dependent cellular immune responses to Plasmodium vivax Duffy binding protein in humans.
Xainli J, Baisor M, Kastens W, Bockarie M, Adams JH, King CL
Journal of immunology (Baltimore, Md. : 1950) 2002 Sep 15;169(6):3200-7
Journal of immunology (Baltimore, Md. : 1950) 2002 Sep 15;169(6):3200-7
Age-dependent cellular immune responses to Plasmodium vivax Duffy binding protein in humans.
Xainli J, Baisor M, Kastens W, Bockarie M, Adams JH, King CL
Journal of immunology (Baltimore, Md. : 1950) 2002 Sep 15;169(6):3200-7
Journal of immunology (Baltimore, Md. : 1950) 2002 Sep 15;169(6):3200-7
Differing influences of virus burden and immune activation on disease severity in secondary dengue-3 virus infections.
Libraty DH, Endy TP, Houng HS, Green S, Kalayanarooj S, Suntayakorn S, Chansiriwongs W, Vaughn DW, Nisalak A, Ennis FA, Rothman AL
The Journal of infectious diseases 2002 May 1;185(9):1213-21
The Journal of infectious diseases 2002 May 1;185(9):1213-21
A HLA-A2 restricted human CTL line recognizes a novel tumor cell expressed p53 epitope.
Würtzen PA, Claesson MH
International journal of cancer 2002 Jun 1;99(4):568-72
International journal of cancer 2002 Jun 1;99(4):568-72
Characterization of CD8(+) effector T cell responses in volunteers immunized with Salmonella enterica serovar Typhi strain Ty21a typhoid vaccine.
Salerno-Goncalves R, Pasetti MF, Sztein MB
Journal of immunology (Baltimore, Md. : 1950) 2002 Aug 15;169(4):2196-203
Journal of immunology (Baltimore, Md. : 1950) 2002 Aug 15;169(4):2196-203
Characterization of CD8(+) effector T cell responses in volunteers immunized with Salmonella enterica serovar Typhi strain Ty21a typhoid vaccine.
Salerno-Goncalves R, Pasetti MF, Sztein MB
Journal of immunology (Baltimore, Md. : 1950) 2002 Aug 15;169(4):2196-203
Journal of immunology (Baltimore, Md. : 1950) 2002 Aug 15;169(4):2196-203
Inhibition of human immunodeficiency virus type 1 gp120 presentation to CD4 T cells by antibodies specific for the CD4 binding domain of gp120.
Hioe CE, Tuen M, Chien PC Jr, Jones G, Ratto-Kim S, Norris PJ, Moretto WJ, Nixon DF, Gorny MK, Zolla-Pazner S
Journal of virology 2001 Nov;75(22):10950-7
Journal of virology 2001 Nov;75(22):10950-7
Schistosoma haematobium-induced urinary tract morbidity correlates with increased tumor necrosis factor-alpha and diminished interleukin-10 production.
King CL, Malhotra I, Mungai P, Wamachi A, Kioko J, Muchiri E, Ouma JH
The Journal of infectious diseases 2001 Nov 1;184(9):1176-82
The Journal of infectious diseases 2001 Nov 1;184(9):1176-82
Adaptive immune responses of patients with asthma to the attachment (G) glycoprotein of respiratory synctial virus.
Hancock GE, Scheuer CA, Sierzega R, Pryharski KS, McBride JT, Watelet LF, Tebbey PW, Smith JD
The Journal of infectious diseases 2001 Dec 15;184(12):1589-93
The Journal of infectious diseases 2001 Dec 15;184(12):1589-93
A T cell clone's avidity is a function of its activation state.
Hesse MD, Karulin AY, Boehm BO, Lehmann PV, Tary-Lehmann M
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1353-61
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1353-61
A T cell clone's avidity is a function of its activation state.
Hesse MD, Karulin AY, Boehm BO, Lehmann PV, Tary-Lehmann M
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1353-61
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1353-61
IL-10 secretion and sensitivity in normal human intestine and inflammatory bowel disease.
Gasche C, Bakos S, Dejaco C, Tillinger W, Zakeri S, Reinisch W
Journal of clinical immunology 2000 Sep;20(5):362-70
Journal of clinical immunology 2000 Sep;20(5):362-70
IL-10 secretion and sensitivity in normal human intestine and inflammatory bowel disease.
Gasche C, Bakos S, Dejaco C, Tillinger W, Zakeri S, Reinisch W
Journal of clinical immunology 2000 Sep;20(5):362-70
Journal of clinical immunology 2000 Sep;20(5):362-70
Immune responses to hepatitis C and non-hepatitis C antigens in hepatitis C virus infected and HIV-1 coinfected patients.
Valdez H, Anthony D, Farukhi F, Patki A, Salkowitz J, Heeger P, Peterson DL, Post AB, Asaad R, Lederman MM
AIDS (London, England) 2000 Oct 20;14(15):2239-46
AIDS (London, England) 2000 Oct 20;14(15):2239-46
Immune responses to hepatitis C and non-hepatitis C antigens in hepatitis C virus infected and HIV-1 coinfected patients.
Valdez H, Anthony D, Farukhi F, Patki A, Salkowitz J, Heeger P, Peterson DL, Post AB, Asaad R, Lederman MM
AIDS (London, England) 2000 Oct 20;14(15):2239-46
AIDS (London, England) 2000 Oct 20;14(15):2239-46
CD40-ligand (CD154) gene therapy for chronic lymphocytic leukemia.
Wierda WG, Cantwell MJ, Woods SJ, Rassenti LZ, Prussak CE, Kipps TJ
Blood 2000 Nov 1;96(9):2917-24
Blood 2000 Nov 1;96(9):2917-24
Naturally developing memory T cell xenoreactivity to swine antigens in human peripheral blood lymphocytes.
Hartig CV, Haller GW, Sachs DH, Kuhlenschmidt S, Heeger PS
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2790-6
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2790-6
Helminth- and Bacillus Calmette-Guérin-induced immunity in children sensitized in utero to filariasis and schistosomiasis.
Malhotra I, Mungai P, Wamachi A, Kioko J, Ouma JH, Kazura JW, King CL
Journal of immunology (Baltimore, Md. : 1950) 1999 Jun 1;162(11):6843-8
Journal of immunology (Baltimore, Md. : 1950) 1999 Jun 1;162(11):6843-8
Pretransplant frequency of donor-specific, IFN-gamma-producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes.
Heeger PS, Greenspan NS, Kuhlenschmidt S, Dejelo C, Hricik DE, Schulak JA, Tary-Lehmann M
Journal of immunology (Baltimore, Md. : 1950) 1999 Aug 15;163(4):2267-75
Journal of immunology (Baltimore, Md. : 1950) 1999 Aug 15;163(4):2267-75
T-cell anergy induced by clonotype-specific antibodies: modulation of an autoreactive human T-cell clone in vitro.
Steenbakkers PG, Boots AM, Rijnders AW
Immunology 1999 Apr;96(4):586-94
Immunology 1999 Apr;96(4):586-94
T-cell anergy induced by clonotype-specific antibodies: modulation of an autoreactive human T-cell clone in vitro.
Steenbakkers PG, Boots AM, Rijnders AW
Immunology 1999 Apr;96(4):586-94
Immunology 1999 Apr;96(4):586-94
Donor-specific stimulation of peripheral blood mononuclear cells from recipients of orthotopic liver transplants is associated, in the absence of rejection, with type-2 cytokine production.
Chung SW, Yoshida EM, Cattral MS, Hu Y, Gorczynski RM
Immunology letters 1998 Sep;63(2):91-6
Immunology letters 1998 Sep;63(2):91-6
Down-regulation of CD1 on antigen-presenting cells by infection with Mycobacterium tuberculosis.
Stenger S, Niazi KR, Modlin RL
Journal of immunology (Baltimore, Md. : 1950) 1998 Oct 1;161(7):3582-8
Journal of immunology (Baltimore, Md. : 1950) 1998 Oct 1;161(7):3582-8
Increased interleukin-4 and interleukin-5 production in response to Schistosoma haematobium adult worm antigens correlates with lack of reinfection after treatment.
Medhat A, Shehata M, Bucci K, Mohamed S, Dief AD, Badary S, Galal H, Nafeh M, King CL
The Journal of infectious diseases 1998 Aug;178(2):512-9
The Journal of infectious diseases 1998 Aug;178(2):512-9
Increased interleukin-4 and interleukin-5 production in response to Schistosoma haematobium adult worm antigens correlates with lack of reinfection after treatment.
Medhat A, Shehata M, Bucci K, Mohamed S, Dief AD, Badary S, Galal H, Nafeh M, King CL
The Journal of infectious diseases 1998 Aug;178(2):512-9
The Journal of infectious diseases 1998 Aug;178(2):512-9
Ligation of TAPA-1 (CD81) or major histocompatibility complex class II in co-cultures of human B and T lymphocytes enhances interleukin-4 synthesis by antigen-specific CD4+ T cells.
Secrist H, Levy S, DeKruyff RH, Umetsu DT
European journal of immunology 1996 Jul;26(7):1435-42
European journal of immunology 1996 Jul;26(7):1435-42
IL-12 synthesis by human Langerhans cells.
Kang K, Kubin M, Cooper KD, Lessin SR, Trinchieri G, Rook AH
Journal of immunology (Baltimore, Md. : 1950) 1996 Feb 15;156(4):1402-7
Journal of immunology (Baltimore, Md. : 1950) 1996 Feb 15;156(4):1402-7
Interleukin 12 synergizes with B7/CD28 interaction in inducing efficient proliferation and cytokine production of human T cells.
Kubin M, Kamoun M, Trinchieri G
The Journal of experimental medicine 1994 Jul 1;180(1):211-22
The Journal of experimental medicine 1994 Jul 1;180(1):211-22
Interleukin 12 synergizes with B7/CD28 interaction in inducing efficient proliferation and cytokine production of human T cells.
Kubin M, Kamoun M, Trinchieri G
The Journal of experimental medicine 1994 Jul 1;180(1):211-22
The Journal of experimental medicine 1994 Jul 1;180(1):211-22
Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells.
D'Andrea A, Aste-Amezaga M, Valiante NM, Ma X, Kubin M, Trinchieri G
The Journal of experimental medicine 1993 Sep 1;178(3):1041-8
The Journal of experimental medicine 1993 Sep 1;178(3):1041-8
Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells.
D'Andrea A, Aste-Amezaga M, Valiante NM, Ma X, Kubin M, Trinchieri G
The Journal of experimental medicine 1993 Sep 1;178(3):1041-8
The Journal of experimental medicine 1993 Sep 1;178(3):1041-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IFN Gamma was performed by loading indicated amount of Human IFN Gamma recombinant protein in reducing sample buffer and 8 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. IFN Gamma was detected at ~16 kDa using a IFN Gamma rat monoclonal biotin conjugate antibody (Product # M701) at a concentration of 2 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody (Product # A28177) at a dilution of 1:2,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078) and the myECL Imager (Product # 62236).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of IFN-gamma on PHA-M treated Jurkat cells. Cells were fixed with 4% formaldehyde for 30 min on ince and permeabilized with IC permeabilization buffer (Product # PB001). After incubation with blocking buffer (Product # 37525) for 30 min on ice, cells were then stained with IFN-gamma mouse monoclonal antibody (Product # M701) at 1:100 dilution with IC permeabilization buffer for 30 min on ice. After washing with ice-cold IC permeabilization buffer for 3 times, the cells were stained with DyLight 488 goat anti-mouse secondary antibody (Product # 35502) for 30 min on ice. A representative 10,000 cells were acquired for each sample.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Increased responses to NS3 protein inHCV-infected patients only after in vitro costimulation with IL-7/IL-15 oranti-CD28. PBMCs of 10 HCV-infectedpatients were tested in IFN- gamma ELISPOT assays for their recall responses to NS3protein and Pepset 4 peptide pool in the absence (a) or presence of IL-7/IL-15(b), IFN- alpha (c), anti-ICOS (d), or CD28 (e). For each individual, the mean spot count of duplicate wells isshown. Horizontal bars indicate themeans of all individual responses to the respective antigens. P values are given for means that aresignificantly different from baseline values in (a). Symbols in (a) also apply to (b)-(e).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 In vitro costimulation has no effect onantigen-specific T cell responses in HIV-infected patients. PBMCs of 10 HIV-infected patients were tested in IFN- gamma ELISPOT assays for their recall responses to HIV peptide pools 1, 2,and 3 (as described in Section 2 )in the absence (a) or presence of IL-7/IL-15 (b), IFN- alpha (c), anti-ICOS (d), orCD28 (e). For each individual, the meanspot count of duplicate wells is shown. Horizontal bars indicate the means of all individual responses to therespective antigens. Symbols in (a) alsoapply to (b)-(e).
