AF961

antibody from Novus Biologicals

Targeting: IFNG

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF961

Provider

Novus Biologicals

Product name

Goat Polyclonal IFN-gamma Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects rhesus macaque IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 30% cross-reactivity with recombinant human IFN-gamma is observed and less than 0.1% cross-reactivity with recombinant mouse IFN-gamma , recombinant rat IFN-gamma , recombinant porcine IFN-gamma , recombinant canine IFN-gamma , recombinant cotton rat IFN-gamma , and recombinant feline IFN-gamma is observed.

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Primate IFN-gamma Antibody. Recombinant Rhesus Macaque IFN-gamma (Catalog # 961-RM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rhesus Macaque IFN-gamma (200 ng/mL) is neutralized (green line) by increasing concentrations of Rhesus Macaque IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961). The ND₅₀ is typically 1.5-5.0 µg/mL.