AF1586

antibody from Novus Biologicals

Targeting: IFNG

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1586

Provider

Novus Biologicals

Product name

Goat Polyclonal IFN-gamma Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects equine IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant canine IFN-gamma is observed, less than 6% cross-reactivity with recombinant bovine IFN-gamma and recombinant feline IFN-gamma is observed, and less than 0.2% cross-reactivity with recombinant human IFN-gamma , recombinant mouse IFN-gamma , recombinant rat IFN-gamma , recombinant porcine IFN-gamma , recombinant rhesus macaque IFN-gamma , and recombinant cotton rat IFN-gamma is observed.

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Equine IFN-gamma Antibody. Recombinant Equine IFN-gamma (Catalog # 1586-HG) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Equine IFN-gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586). The ND₅₀ is typically 0.25-1.25 µg/mL.